2. 重组蛋白
  3. Cytokines and Growth Factors
  4. Ephrin/Eph Family
  5. Ephrins
  6. Ephrin B2
  7. Ephrin-B2/EFNB2 蛋白, Mouse (HEK293, His)

Ephrin-B2/EFNB2 蛋白, Mouse (HEK293, His)

目录号: HY-P73018
产品使用指南

Ephrin-B2/EFNB2 蛋白是 Eph 受体的跨膜配体，介导双向信号传导并与 EPHA4、EPHA3 和 EPHB4 结合。它调节细胞粘附、迁移、心脏形态发生、血管生成和轴突定向。它还与 PDZRN3 相互作用。Ephrin-B2/EFNB2 蛋白, Mouse (HEK293, His) 是重组的 Ephrin-B2/EFNB2 蛋白，由 HEK293 表达，带有 C-His 标签。Ephrin-B2/EFNB2 蛋白, Mouse (HEK293, His) 全长 204 个氨基酸，分子量为 30-40 kDa。

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描述

Ephrin-B2/EFNB2 Protein is a transmembrane ligand for Eph receptors, mediating bidirectional signaling and binding to EPHA4, EPHA3, and EPHB4. It regulates cell adhesion, migration, heart morphogenesis, angiogenesis, and axon orientation. It also interacts with PDZRN3. Ephrin-B2/EFNB2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Ephrin-B2/EFNB2 protein, expressed by HEK293 , with C-His labeled tag. The total length of Ephrin-B2/EFNB2 Protein, Mouse (HEK293, His) is 204 a.a., with molecular weight of 30-40 kDa.
研究背景

Ephrin-B2/EFNB2 蛋白是 Eph 受体的细胞表面跨膜配体，Eph 受体是受体酪氨酸激酶家族，在神经元、血管和上皮发育中发挥着至关重要的作用。它与邻近细胞的 Eph 受体结合，产生接触依赖性的双向信号传导。这种相互作用触发受体下游的正向信号传导和肝配蛋白配体下游的反向信号传导。Ephrin-B2/EFNB2 与受体酪氨酸激酶（例如 EPHA4、EPHA3 和 EPHB4）混杂结合。它与 EPHB4 配合调节细胞粘附、迁移、心脏形态发生和血管生成。此外，它还参与引导纵向突出轴突的方向并与 PDZRN3 相互作用（通过相似性）。
种属

Mouse
表达系统

HEK293
标签

C-His
蛋白编号

P52800 (R29-A232)
基因 ID
蛋白结构
N-term
EFNB2 (R29-A232)
Accession # P52800
His
C-term
同用名
Ephrin-B2; LERK-5; HTK-L; EFNB2; EPLG5
氨基酸序列

MAMARSRRDSVWKYCWGLLMVLCRTAISRSIVLEPIYWNSSNSKFLPGQGLVLYPQIGDKLDIICPKVDSKTVGQYEYYKVYMVDKDQADRCTIKKENTPLLNCARPDQDVKFTIKFQEFSPNLWGLEFQKNKDYYIISTSNGSLEGLDNQEGGVCQTRAMKILMKVGQDASSAGSARNHGPTRRPELEAGTNGRSSTTSPFVKPNPGSSTDGNSAGHSGNNLLGSEVALFA

分子量

30-40 kDa
纯度

Greater than 95% as determined by reducing SDS-PAGE
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

Ephrin-B2/EFNB2 Protein, Mouse (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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