Epiregulin 蛋白, Human

Epiregulin Protein, Human is a growth regulator and a member of the epidermal growth factor (EGF) family. Epiregulin is a tumor growth-inhibitory factor inducing morphological changes in human epidermoid carcinoma HeLa cells.

人类上皮调节蛋白基因编码一个由163个残基组成的推定跨膜前体，其内部片段含有一个EGF样结构域，其结构组织与与EGF受体结合的EGF家族其他成员相似。上皮调节蛋白具有不同于 EGF 家族经典成员、EGF 和转化生长因子 α 的某些特征，包括对几种正常细胞的促有丝分裂反应以及与表皮样癌 A431 细胞上的 EGF 受体结合。Northern blot分析显示，人上皮调节蛋白主要在正常组织的外周血巨噬细胞和胎盘上表达，在各类肿瘤细胞系中，在上皮性肿瘤细胞系上表达最高。上皮调节蛋白参与某些生理过程，例如正常细胞生长的维持或发展以及癌症的进展^[1]。

1. The ED₅₀ is <2 ng/mL as measured by murine Ballb/c 3T3 cells, corresponding to a specific activity of >5 × 10⁵ units/mg.
2. Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED₅₀ for this effect is 0.3417 ng/mL, corresponding to a specific activity is 2.927×10^6 units/mg.

Human

E. coli

Tag Free

O14944 (V60-L108)

2069  [NCBI]

VAQVSITKCSSDMNGYCLHGQCIYLVDMSQNYCRCEVGYTGVRCEHFFL

Approximately 5.6 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Toyoda H, et al. Distribution of mRNA for human epiregulin, a differentially expressed member of the epidermal growth factor family. Biochem J. 1997 Aug 15;326 ( Pt 1):69-75.  [Content Brief]