ERG11 蛋白, Saccharomyces cerevisiae (GST)

ERG11 Proteinas is a sterol 14α-demethylase, a key enzyme in the ergosterol biosynthetic pathway, particularly in the late pathway that occurs in the endoplasmic reticulum membrane. ERG11 catalyzes the three-step removal of the 14α-methyl group from lanosterol, releasing it as a formate, resulting in the conversion of the sterol into a key intermediate in ergosterol biosynthesis. ERG11 Protein, Saccharomyces cerevisiae (GST) is the recombinant ERG11 protein, expressed by E. coli , with N-GST labeled tag. The total length of ERG11 Protein, Saccharomyces cerevisiae (GST) is 20 a.a., with molecular weight of ~29.1 kDa.

ERG11蛋白是一种甾醇14α-去甲基酶，是麦角甾醇生物合成途径第三个模块中的关键酶，在真菌膜中发挥着关键作用，其中麦角甾醇是具有多种功能的主要甾醇成分。第三个模块被称为晚期途径，涉及主要在内质网 (ER) 膜中发生的连续反应，最终导致麦角甾醇的合成。ERG11 以羊毛甾醇为起始原料，催化三步氧化去除甾醇的 14α-甲基 (C-32)，并以甲酸盐的形式释放出来。该过程导致甾醇转化为 4,4-二甲基-5α-胆甾醇-8,14,24-三烯-3β-醇，这是麦角甾醇生物合成的关键中间体。有趣的是，ERG11 表现出对酵母非固有底物（包括 eburicol 和 24,25-二氢羊毛甾醇）去甲基化的能力，突出了其在甾醇修饰方面的多功能性。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Others

E. coli

N-GST

P10614 (M1-S20)

856398  [NCBI]

MSATKSIVGEALEYVNIGLS

Approximately 29.1 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.