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  3. ERH 蛋白, Human (N-His)

ERH 蛋白, Human (N-His)

目录号: HY-P74169
COA 产品使用指南

ERH 蛋白参与调节细胞周期,并已被证明参与细胞分裂和增殖过程。在结构上形成同二聚体,这表明潜在的功能意义。ERH 蛋白, Human (N-His) 是重组的 ERH 蛋白,由 E. coli 表达,带有 N-His 标签。ERH 蛋白, Human (N-His) 全长 104 个氨基酸,分子量约为 ~15 kDa。

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描述

ERH proteins are involved in regulating the cell cycle and have been shown to be involved in cell division and proliferation processes. Structurally forming homodimers, it suggests potential functional significance. ERH Protein, Human (N-His) is the recombinant human-derived ERH protein, expressed by E. coli , with N-His labeled tag. The total length of ERH Protein, Human (N-His) is 104 a.a., with molecular weight of ~15 kDa.

研究背景

ERH 蛋白被认为在细胞周期的调节中发挥作用,表明它可能参与控制细胞分裂和增殖的复杂过程。在结构上,它形成同型二聚体,这一特征表明了其分子排列和潜在的功能意义。此外,ERH 与 POLDIP3 相互作用,并且是甲基小体的组成部分,甲基小体是一种 20S 复合物,包括 CLNS1A/pICln、PRMT5/SKB1、WDR77/MEP50、PRMT1 和 ERH。研究中发现,这种复合物暗示 ERH 参与蛋白质甲基化过程。此外,ERH 与 CHTOP 相互作用,进一步表明其参与多种分子途径,并强调其在细胞机制中的多方面作用。

种属

Human

表达系统

E. coli

标签

N-His

蛋白编号

P84090 (M1-K104)

基因 ID
蛋白结构
N-term
His
ERH (M1-K104)
Accession # P84090
C-term
同用名
Enhancer of rudimentary homolog; ERH
分子量

Approximately 15 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris, 300mM NaCl, 5% trehalose, 5% mannitol and 0.01% Tween80, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

ERH Protein, Human (N-His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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