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  3. ERP27 蛋白, Human (HEK293, His)

ERP27 蛋白, Human (HEK293, His)

目录号: HY-P70891
产品使用指南

ERP27蛋白具有独特的作用,它可以特异性结合未折叠蛋白,并通过其C端结构域的疏水口袋将蛋白二硫键异构酶PDIA3招募到底物上。这种相互作用意味着 ERP27 参与了应激反应的展开并解决了细胞蛋白质稳态的挑战。ERP27 蛋白, Human (HEK293, His) 是重组的 ERP27 蛋白,由 HEK293 表达,带有 C-6*His 标签。ERP27 蛋白, Human (HEK293, His) 全长 248 个氨基酸,分子量为 32-38 kDa。

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描述

The ERP27 protein has a unique role in that it can specifically bind unfolded proteins and recruit the protein disulfide isomerase PDIA3 to the substrate through the hydrophobic pocket in its C-terminal domain. This interaction implies that ERP27 is involved in the unfolding stress response and resolves challenges in cellular proteostasis. ERP27 Protein, Human (HEK293, His) is the recombinant human-derived ERP27 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of ERP27 Protein, Human (HEK293, His) is 248 a.a., with molecular weight of 32-38 kDa.

研究背景

ERP27 是一种具有独特作用的蛋白质,与未折叠蛋白质表现出特异性结合,并具有将蛋白质二硫键异构酶 PDIA3 募集到未折叠底物的潜力。这种与蛋白质底物的结合是通过位于 ERP27 C 末端结构域的疏水口袋来促进的。该蛋白质与未折叠底物的相互作用表明它可能参与未折叠应激反应,这意味着在解决蛋白质稳态挑战的细胞机制中发挥作用。此外,ERP27 与 PDIA3 相互作用,强调其与参与蛋白质折叠和质量控制的复杂细胞蛋白质网络的关联。ERP27 的特定分子相互作用和结合特性强调了其在细胞应激反应和蛋白质稳态途径中的潜在重要性。

种属

Human

表达系统

HEK293

标签

C-6*His

蛋白编号

Q96DN0 (E26-L273)

基因 ID
蛋白结构
N-term
ERP27 (E26-L273)
Accession # Q96DN0
6*His
C-term
同用名
Endoplasmic Reticulum Resident Protein 27; ER Protein 27; ERp27; ERP27; C12orf46
氨基酸序列

EVEKSSDGPGAAQEPTWLTDVPAAMEFIAATEVAVIGFFQDLEIPAVPILHSMVQKFPGVSFGISTDSEVLTHYNITGNTICLFRLVDNEQLNLEDEDIESIDATKLSRFIEINSLHMVTEYNPVTVIGLFNSVIQIHLLLIMNKASPEYEENMHRYQKAAKLFQGKILFILVDSGMKENGKVISFFKLKESQLPALAIYQTLDDEWDTLPTAEVSVEHVQNFCDGFLSGKLLKENRESEGKTPKVEL

分子量

32-38 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

ERP27 Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
ERP27 Protein, Human (HEK293, His)
目录号:
HY-P70891
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