ETFR-2/TEAD-4 蛋白, Mouse

ETFR-2/TEAD-4 protein is a key transcription factor that tightly regulates the Hippo signaling pathway and is an integral component of organ size control and tumor suppression. In this pathway, it mediates a kinase cascade involving MST1/MST2, SAV1, LATS1/2, and MOB1, leading to the inactivation of YAP1 and WWTR1/TAZ. ETFR-2/TEAD-4 Protein, Mouse is the recombinant mouse-derived ETFR-2/TEAD-4 protein, expressed by E. coli , with tag free.

ETFR-2/TEAD-4 是一种关键的转录因子，在 Hippo 信号通路中发挥着关键作用，该通路通过增殖和凋亡的调节与器官大小控制和肿瘤抑制密切相关。该途径涉及激酶级联，其中 MST1/MST2 与其调节蛋白 SAV1 复合，磷酸化并激活与其调节蛋白 MOB1 复合的 LATS1/2。随后，MOB1 磷酸化并灭活 YAP1 癌蛋白和 WWTR1/TAZ。ETFR-2/TEAD-4 通过介导 YAP1 和 WWTR1/TAZ 的基因表达发挥作用，从而调节关键的细胞过程，例如增殖、迁移和上皮间质转化 (EMT) 诱导。该蛋白与 Sph 和 GT-IIC“enhansons”(5'-GTGGAATGT-3') 特异性且非合作性结合并激活转录。此外，它还与 M-CAT 基序相互作用。虽然 ETFR-2/TEAD-4 可能在骨骼肌的胚胎发育中发挥作用，但它还与 WWTR1/TAZ 和 YAP1 相互作用，突出了其在调控网络中的多方面参与。

Mouse

E. coli

Tag Free

Q62296-1 (R210-E427)

21679  [NCBI]

RSIASSKLWMLEFSAFLERQQDPDTYNKHLFVHISQSSPSYSDPYLETVDIRQIYDKFPEKKGGLKELFERGPSNAFFLVKFWADLNTNIDDEGSAFYGVSSQYESPENMIITCSTKVCSFGKQVVEKVETEYARYENGHYLYRIHRSPLCEYMINFIHKLKHLPEKYMMNSVLENFTILQVVTNRDTQETLLCIAYVFEVSASEHGAQHHIYRLVKE

Approximately 25.7 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized after extensive dialysis against solution in 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.