Exodus-2/CCL21 蛋白, Human (sf9)

Exodus-2/CCL21 Protein, Human (sf9) is a homeostatic lymphoid chemokine that contributes to the entry of T cells and dendritic cells into the lymphoid T-zone. It acts through chemokine receptors CCR7 and CXCR3 to promote fibrogenic and inflammatory cytokine production. Exodus-2/CCL21 Protein, Human (sf9) is a recombinant human Exodus-2/CCL21 (S24-P134) expressed by Sf9 insect cells^[1].

CCL21，也称为 exodus-2 和次级淋巴趋化因子 (SLC)，是属于 CC 趋化因子家族的一种小细胞因子，位于人类基因组中的第 9 号染色体上。它与糖胺聚糖 (GAG) 结合并固定在内皮细胞的表面。作为一种趋化因子，CCL21 抑制造血并刺激趋化性，并且在体外对胸腺细胞和活化的 T 细胞具有趋化性，但对 B 细胞、巨噬细胞或中性粒细胞没有趋化性。同时，CCL21是T细胞迁移和粘附的强效刺激剂，与T细胞上的糖蛋白PSGL-1结合，促进T细胞迁移至次级淋巴器官。CCL21 可通过趋化因子受体 CCR7 和 CXCR3 发挥作用。其中，CCR7是一种GPCR，通常由T细胞亚群中央记忆细胞、胸腺T细胞、B细胞、成熟DC和其他稀有细胞亚群表达。ccl21 可作为中枢神经系统中的小胶质细胞激活剂，仅在濒危或机械损伤的神经元中表达^[1][2]。

CCL21(.1 µg/mL, 2 h) can interact with polysialic acid to regulate the migratory capacity of human dendritic cells^[3].

Measured by its binding ability in a functional ELISA. Immobilized Exodus-2/CCL21 Protein, Human (sf9) at 2 μg/mL (100 μl/well) can bind human IGFBP7 with a linear range of 0.16-4 μg/mL.

Human

Sf9 insect cells

Tag Free

O00585 (S24-P134)

6366  [NCBI]

MAQSLALSLLILVLAFGIPRTQGSDGGAQDCCLKYSQRKIPAKVVRSYRKQEPSLGCSIPAILFLPRKRSQAELCADPKELWVQQLMQHLDKTPSPQKPAQGCRKDRGASKTGKKGKGSKGCKRTERSQTPKGP

Approximately 18 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 40 mM Tris, 0.3 M NaCl, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Balsam Rizeq, et al. The Role of CCL21/CCR7 Chemokine Axis in Breast Cancer Progression. Cancers (Basel). 2020 Apr 23;12(4):1036.  [Content Brief]

  [2]. Knut Biber, et al. Neuronal CCL21 up-regulates microglia P2X4 expression and initiates neuropathic pain development. EMBO J. 2011 May 4;30(9):1864-73.  [Content Brief]

  [3]. Marieke Bax, et al. Interaction of polysialic acid with CCL21 regulates the migratory capacity of human dendritic cells. PLoS One. 2009 Sep 14;4(9):e6987.  [Content Brief]