1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. Exopolyphosphatase/PPX 蛋白, E.coli (His)

Exopolyphosphatase/PPX 蛋白, E.coli (His)

目录号: HY-P71502
COA 产品使用指南

外多磷酸酶/PPX 蛋白是一种关键酶,通过催化正磷酸盐的持续释放来降解无机多磷酸盐 (polyP)。这种活性对于调节细胞多聚蛋白水平至关重要,并且与多种生物过程有关。该蛋白质在多聚磷降解中的作用有助于维持磷酸盐稳态,并且是涉及多聚磷的复杂细胞过程网络的组成部分。Exopolyphosphatase/PPX 蛋白, E.coli (His) 是重组的 外多磷酸酶/PPX 蛋白,由 E. coli 表达,带有 N-6*His 标签。Exopolyphosphatase/PPX 蛋白, E.coli (His) 全长 512 个氨基酸,分子量约为 ~62.0 kDa。

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10 μg ¥2360 In-stock
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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

Exopolyphosphatase/PPX protein, a key enzyme, degrades inorganic polyphosphates (polyP) by catalyzing the processive release of orthophosphate. This activity is crucial for regulating cellular polyP levels and is implicated in various biological processes. The protein's role in polyP degradation contributes to maintaining phosphate homeostasis and is integral to the intricate network of cellular processes involving polyP. Exopolyphosphatase/PPX Protein, E.coli (His) is the recombinant E. coli-derived Exopolyphosphatase/PPX protein, expressed by E. coli , with N-6*His labeled tag. The total length of Exopolyphosphatase/PPX Protein, E.coli (His) is 512 a.a., with molecular weight of ~62.0 kDa.

研究背景

外多磷酸酶/PPX 蛋白是参与无机多磷酸盐 (polyP) 降解的关键酶。作为外切多磷酸酶,它催化正磷酸盐从多磷酸链末端持续释放。这种酶活性对于调节细胞多聚蛋白水平至关重要,并且与多种生物过程有关。外多磷酸酶/PPX 蛋白在 PolyP 降解中的作用有助于维持细胞内磷酸盐稳态,并且是 PolyP 参与的复杂细胞过程网络的组成部分。

生物活性

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

种属

E.coli

表达系统

E. coli

标签

N-6*His

蛋白编号

P0AFL7 (P2-A513)

基因 ID

57732396

蛋白结构
N-term
6*His
PPX (P2-A513)
Accession # P0AFL7
C-term
同用名
ppx; c3020; Exopolyphosphatase; ExopolyPase; EC 3.6.1.11
氨基酸序列

PIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQRVHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFLKRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPILVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASGTIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLAILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQARRVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNSDLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEESTPEIAA

分子量

Approximately 62.0 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

Exopolyphosphatase/PPX Protein, E.coli (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Exopolyphosphatase/PPX Protein, E.coli (His)
目录号:
HY-P71502
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