Fc gamma RIIB/CD32b 蛋白, Cynomolgus (HEK293, His)

Fc γ RIIB/CD32b proteins exhibit low affinity as complexes or receptors that aggregate the immunoglobulin γFc region. ITIM phosphorylated by Fc γ RIIB/CD32b recruits inositol phosphatases SHIP1 and SHIP2, inhibits Ras activation, down-regulates MAPK activity, reduces PLC-γ function, and results in PKC activation. Inhibition of MAP kinase pathway and anti-apoptotic kinase Akt inhibit cell proliferation and survival. Fc gamma RIIB/CD32b Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived Fc gamma RIIB/CD32b protein, expressed by HEK293 , with C-His labeled tag.

Fc γ RIIB/CD32b 蛋白作为复合物或聚集免疫球蛋白 γ Fc 区的受体，表现出低亲和力。它在多种效应和调节功能中起关键作用，包括免疫复合物的吞噬和 B 细胞抗体产生的调节。与该受体结合后，通过 B 细胞 (BCR)、T 细胞 (TCR) 或另一种 Fc 受体上的抗原受体触发细胞激活的先前状态下调。IIB1 亚型不能介导内吞作用或吞噬作用，而 IIB2 亚型不诱导吞噬作用。Fc γ RIIB/CD32b 与INPP5D/SHIP1、FGR 和 LYN 相互作用。Fc γ RIIB/CD32b 磷酸化的 ITIM 募集肌醇磷酸酶 SHIP1 和SHIP2，抑制 Ras 活化，下调 MAPK 活性，降低 PLCγ 功能，导致 PKC 活化降低。抑制 MAP 激酶通路和抗凋亡激酶 Akt 抑制细胞增殖和存活。

Measured by its binding ability in a functional ELISA.Immobilized cynoCD32B-His at 10 μg/mL (100 μl/well) can bind biotinylated human IgG1, The EC₅₀ of biotinylated human IgG1 is 0.17-0.41 μg/mL.

Cynomolgus

HEK293

C-His

Q8SPW3/AAL92097.1 (A45-P217)

102139530  [NCBI]

28-33 kDa

Greater than 95% as determined by reducing SDS-PAGE

溶液

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.