Fc gamma RIIB/CD32b 蛋白, Human (HEK293, His)

Fc gamma RIIB/CD32b protein is a low-affinity receptor for immunoglobulin gamma and plays a key role in immune responses. It mediates phagocytosis of immune complexes and regulates antibody production by B cells. Fc gamma RIIB/CD32b Protein, Human (HEK293, His) is the recombinant human-derived Fc gamma RIIB/CD32b protein, expressed by HEK293 , with C-6*His labeled tag.

Fc gamma RIIB/CD32b 蛋白充当复合或聚集的免疫球蛋白 gamma Fc 区的受体，显示出低亲和力。它在各种效应器和调节功能中发挥着关键作用，包括免疫复合物的吞噬作用和 B 细胞抗体产生的调节。与该受体结合后，B 细胞 (BCR)、T 细胞 (TCR) 或其他 Fc 受体上的抗原受体触发先前的细胞激活状态下调。值得注意的是，异构体 IIB1 不能介导内吞作用或吞噬作用，而异构体 IIB2 不诱导吞噬作用。Fc gamma RIIB/CD32b 与 INPP5D/SHIP1、FGR 和 LYN 相互作用。

Measured by its binding ability in a functional ELISA. Immobilized Human FcγRIIB, at 2 μg/mL (100 μL/well) can bind Biotinylated Human IgG1 protein. The ED₅₀ for this effect is 753.3 ng/mL, corresponding to a specific activity is 1327.5 Unit/mg.

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  Measured by its binding ability in a functional ELISA. Immobilized Human FcγRIIB, at 2 μg/mL (100 μL/well) can bind Biotinylated Human IgG1 protein. The ED₅₀ for this effect is 753.3 ng/mL, corresponding to a specific activity is 1327.5 Unit/mg.

Human

HEK293

C-6*His

P31994-1 (T43-P217)

2213  [NCBI]

TPAAPPKAVLKLEPQWINVLQEDSVTLTCRGTHSPESDSIQWFHNGNLIPTHTQPSYRFKANNNDSGEYTCQTGQTSLSDPVHLTVLSEWLVLQTPHLEFQEGETIVLRCHSWKDKPLVKVTFFQNGKSKKFSRSDPNFSIPQANHSHSGDYHCTGNIGYTLYSSKPVTITVQAP

25-30 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2-7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.