Fc gamma RIIIA/CD16a 蛋白, Rat (HEK293, C-His)

Fc γ RIIIA/CD16a is a receptor for the invariant Fc fragment of immunoglobulin γ (IgG). Fc γ RIIIA/CD16a regulates NK cell survival and proliferation and prevents NK cell progenitor cell apoptosis. Fc γ RIIIA/CD16a plays a role in mediating the anti-tumor activity of therapeutic antibodies by triggering TNFA-dependent ADCC, which promotes the entry of the virus into bone marrow cells during secondary infection and subsequent viral replication through antibody-dependent enhancement (ADE). Fc gamma RIIIA/CD16a Protein, Rat (HEK293, C-His) is the recombinant rat-derived Fc gamma RIIIA/CD16a, expressed by HEK293 , with C-His labeled tag. The total length of Fc gamma RIIIA/CD16a Protein, Rat (HEK293, C-His) is 179 a.a.,

Fc γ RIIIA/CD16a 蛋白是免疫球蛋白 γ (IgG) 不变 Fc 片段的受体，在结合细胞表面显示的聚集抗原-IgG复合物时被最佳激活，启动抗体依赖性细胞毒性 (ADCC)。Fc γ RIIIA/CD16a 介导 IgG 效应作用于自然杀伤 (NK) 细胞，结合感染过程中产生的抗原-IgG 复合物，触发 NK 细胞依赖性细胞因子的产生和脱颗粒。Fc γ RIIIA/CD16a 在产生记忆样适应性 NK 细胞中起着至关重要的作用，调节 NK细胞存活、增殖，阻止 NK 细胞祖细胞凋亡。Fc γ RIIIA/CD16a 在介导治疗性抗体的抗肿瘤活性中发挥作用，在 IgG 包被的肿瘤细胞中触发 TNFA 依赖性 ADCC，并在对聚焦的 IgG 的反应中增强 ADCC。Fc γ RIIIA/CD16a 通过抗体依赖性增强 (ADE) 机制参与发病，促进病毒在继发性感染期间进入骨髓细胞并随后进行病毒复制。

Measured by its binding ability in a functional ELISA. Immobilized Rat CD16a protein at 10 μg/mL (100 μL/well) can bind Human IgG1. The ED₅₀ for this effect is 93.49 ng/mL.

Rat

HEK293

C-His

XP_008767961.1 (L22-P200)

304966

LQKAVVILDPEWVRVLEEDCVILRCQGTFSPEDNSTKWFHNKSLISHQDANYVIQSARVKDSGMYRCQTAFSALSDPVQLDVHADWLLLQTTKRLFQEGDPIRLRCHSWRNTPVFKVTYLQNGKGKKYFHRNSELSISKATHADSGSYFCRGIIGRNNISSASLQISIGDPTSPSSFLP

Approximately 27-32 kDa due to the glycosylation

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.