1. 重组蛋白
  2. Fc Receptors Biotinylated Proteins
  3. FcRn
  4. FCGRT-B2M Heterodimer 蛋白, Cynomolgus (Biotinylated, HEK293, His-Avi)

FCGRT-B2M Heterodimer 蛋白, Cynomolgus (Biotinylated, HEK293, His-Avi)

目录号: HY-P76919
产品使用指南

FCGRT 蛋白通过与单体 IgG 结合并促进其从牛奶中的吸收来提供被动免疫。它形成 FcRn-IgG 复合物,通过肠上皮进行转胞吞作用,并将 IgG 释放到血流或间质液中。FCGRT-B2M Heterodimer 蛋白, Cynomolgus (Biotinylated, HEK293, His-Avi) 是重组的蛋白二聚体复合物,包含 FCGRT-B2M 异二聚体蛋白,由 HEK293 表达,带有 C-Avi, C-His 标签。

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描述

The FCGRT protein delivers passive immunity by binding to monomeric IgG and promoting its absorption from milk. It forms an FcRn-IgG complex, which is transcytosis by the intestinal epithelium and releases IgG into the bloodstream or interstitial fluid. FCGRT-B2M Heterodimer Protein, Cynomolgus (Biotinylated, HEK293, His-Avi) is a recombinant protein dimer complex containing cynomolgus-derived FCGRT-B2M Heterodimer protein, expressed by HEK293 , with C-Avi, C-His labeled tag. FCGRT-B2M Heterodimer Protein, Cynomolgus (Biotinylated, HEK293, His-Avi), has molecular weight of ~33.7 & 11.6 KDa, respectively.

研究背景

FCGRT 蛋白是一种细胞表面受体,在将被动体液免疫从母亲转移到新生儿方面发挥着关键作用。它通过与单体免疫球蛋白 γ 的 Fc 区结合并促进其从牛奶中选择性摄取来实现这一点。在肠上皮内,FCGRT-B2M 异二聚体在顶端表面与 IgG 结合,形成 FcRn-IgG 复合物,该复合物在上皮中转胞吞,将 IgG 释放到血流或组织液中。该受体通过回收 IgG 并延长其在循环中的半衰期,在整个生命周期中持续促进有效的体液免疫。从机制上讲,单体 IgG 与内皮细胞和造血细胞酸性内体中的 FCGRT-B2M 结合,使 IgG 能够循环到细胞表面,并释放到循环中。此外,FCGRT-B2M 异二聚体通过与白蛋白(最丰富的循环蛋白)相互作用,在调节白蛋白的稳态中发挥作用。FCGRT-B2M 复合物由两个亚基 p51 和 p14(相当于 β-2-微球蛋白)组成,形成 MHC I 类异二聚体。

种属

Cynomolgus

表达系统

HEK293

标签

C-Avi;C-His

蛋白编号

Q8SPV9 (A24-S297)&Q8SPW0 (I21-M119)

基因 ID
蛋白结构
N-term
FCGRT-B2M Heterodimer ( (I21-M119)
Accession # Q8SPV9
C-term
同用名
FCGRT-B2M Heterodimer Protein; IgG receptor FcRn large subunit p51; Beta-2-microglobulin
分子量

Approximately 33.7&11.6 kDa.

纯度

Greater than 95% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

FCGRT-B2M Heterodimer Protein, Cynomolgus (Biotinylated, HEK293, His-Avi) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
FCGRT-B2M Heterodimer Protein, Cynomolgus (Biotinylated, HEK293, His-Avi)
目录号:
HY-P76919
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