FCGRT-B2M Heterodimer 蛋白, Cynomolgus (HEK293, His)

The FCGRT protein delivers passive immunity by binding to monomeric IgG and promoting its absorption from milk. It forms an FcRn-IgG complex, which is transcytosis by the intestinal epithelium and releases IgG into the bloodstream or interstitial fluid. FCGRT-B2M Heterodimer Protein, Cynomolgus (HEK293, His) is a recombinant protein dimer complex containing cynomolgus-derived FCGRT-B2M Heterodimer protein, expressed by HEK293 , with C-His labeled tag. FCGRT-B2M Heterodimer Protein, Cynomolgus (HEK293, His), has molecular weight of ~35 & 12 kDa, respectively.

FCGRT 蛋白是一种细胞表面受体，在将被动体液免疫从母亲转移到新生儿方面发挥着关键作用。它通过与单体免疫球蛋白 γ 的 Fc 区结合并促进其从牛奶中选择性摄取来实现这一点。在肠上皮内，FCGRT-B2M 异二聚体在顶端表面与 IgG 结合，形成 FcRn-IgG 复合物，该复合物在上皮中转胞吞，将 IgG 释放到血流或组织液中。该受体通过回收 IgG 并延长其在循环中的半衰期，在整个生命周期中持续促进有效的体液免疫。从机制上讲，单体 IgG 与内皮细胞和造血细胞酸性内体中的 FCGRT-B2M 结合，使 IgG 能够循环到细胞表面，并释放到循环中。此外，FCGRT-B2M 异二聚体通过与白蛋白（最丰富的循环蛋白）相互作用，在调节白蛋白的稳态中发挥作用。FCGRT-B2M 复合物由两个亚基 p51 和 p14（相当于 β-2-微球蛋白）组成，形成 MHC I 类异二聚体。

Measured by its binding ability in a functional ELISA. When FCRN-B2M is immobilized at 2 µg/mL (100 µL/well), can bind Biotinylated Human lgG1. The ED₅₀ for this effect is 68.15 ng/mL.

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  Measured by its binding ability in a functional ELISA. When FCRN-B2M is immobilized at 2 µg/mL (100 µL/well),can bind Biotinylated Human lgG1,The ED₅₀ for this effect is 68.15 ng/mL.

Cynomolgus

HEK293

C-His

Q8SPV9 (A24-S297)&Q8SPW0 (I21-M119)

102128913  [NCBI]&101867173  [NCBI]

AESHLSLLYHLTAVSSPAPGTPAFWVSGWLGPQQYLSYDSLRGQAEPCGAWVWENQVSWYWEKETTDLRIKEKLFLEAFKALGGKGPYTLQGLLGCELSPDNTSVPTAKFALNGEEFMNFDLKQGTWGGDWPEALAISQRWQQQDKAANKELTFLLFSCPHRLREHLERGRGNLEWKEPPSMRLKARPGNPGFSVLTCSAFSFYPPELQLRFLRNGMAAGTGQGDFGPNSDGSFHASSSLTVKSGDEHHYCCIVQHAGLAQPLRVELETPAKSS&IQRTPKIQVYSRHPPENGKPNFLNCYVSGFHPSDIEVDLLKNGEKMGKVEHSDLSFSKDWSFYLLYYTEFTPNEKDEYACRVNHVTLSGPRTVKWDRDM

Approximately 35&12 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.