FGF-1 蛋白, Mouse (N-His)

The FGF-1 protein plays a key role in regulating a variety of cellular processes, serving as a potent mitogen and ligand for FGFR1 and integrins. In the presence of heparin, FGF-1 binds to FGFR1, initiating dimerization and autophosphorylation, resulting in multiple signaling cascades. FGF-1 Protein, Mouse (N-His) is the recombinant mouse-derived FGF-1 protein, expressed by E. coli , with N-6*His, N-6*His labeled tag. The total length of FGF-1 Protein, Mouse (N-His) is 140 a.a., with molecular weight of ~17 kDa.

FGF-1 蛋白在调节各种细胞过程中发挥着至关重要的作用，包括细胞存活、分裂、血管生成、分化和迁移。它作为一种有效的有丝分裂原，并作为 FGFR1 和整合素的配体。在肝素存在的情况下，FGF-1 与 FGFR1 结合，通过酪氨酸残基的自磷酸化导致二聚化和激活，酪氨酸残基充当相互作用蛋白质的对接位点。这种激活会触发多个信号级联。FGF-1 还与整合素 ITGAV:ITGB3 结合，与 FGFR1 形成三元复合物，并诱导 PTPN11 募集至该复合物，这对于 FGF-1 信号转导至关重要。它诱导 FGFR1、FRS2、MAPK3/ERK1、MAPK1/ERK2 和 AKT1 的磷酸化和激活，还可以刺激血管生成。FGF-1 与多种受体和蛋白质相互作用，包括 FGFR2、FGFR3、FGFR4、FGFBP1、S100A13、SYT1、LRRC59、CSNKA、CSNKB 和 FIBP。此外，它还与 S100A13 和 SYT1 形成 Cu(2+) 依赖性多蛋白聚集体。虽然 FGF-1 与 LRRC59、CSNKA 和 FIBP 的相互作用似乎是相互排斥的，但 CSNKB 和 FIBP 可能与 FGF-1 协同相互作用。总体而言，FGF-1 通过与各种受体和蛋白质的相互作用在细胞过程中发挥着复杂的作用。

Measured in a cell proliferation assay using NIH-3T3 mouse fibroblast cells.The ED₅₀ for this effect is ≤0.4015 ng/mL, corresponding to a specific activity is ≥2.490×10⁶ units/mg.

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  Measured in a cell proliferation assay using NIH-3T3 mouse fibroblast cells.The ED₅₀ for this effect is 0.4015 ng/mL, corresponding to a specific activity is 2.490×10⁶ units/mg.

Mouse

E. coli

N-6*His

P61148 (F16-D155)

14164  [NCBI]

FNLPLGNYKKPKLLYCSNGGHFLRILPDGTVDGTRDRSDQHIQLQLSAESAGEVYIKGTETGQYLAMDTEGLLYGSQTPNEECLFLERLEENHYNTYTSKKHAEKNWFVGLKKNGSCKRGPRTHYGQKAILFLPLPVSSD

Approximately 17-20 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 6.5 or 50 mM Tris-HCL, 300 mM NaCl, pH 6.5, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.