FGF-12 蛋白, Human (177 a.a)

FGF-12 Protein, Human (177 a.a) is a single polypeptide chain with a molecular mass of 20.4 kDa. FGF12 can play a role in tissues by translocating into cells through the plasma membrane.

成纤维细胞生长因子（FGF）在胚胎发生、血管生成和伤口修复中发挥重要作用，FGF家族目前在人类中有22个成员。FGF12最初被命名为FGF同源因子1（FHF1），通过其与已知FGF的序列同源性来鉴定，与FGF11（FHF2）、FGF12（FHF3）和FGF14（FHF4）具有高度同源性，具有与这些 FGF11 亚家族成员有 58–71% 的氨基酸序列同一性。FGF12与FGF1和FGF2具有结构相似性，因为它缺乏经典信号序列并含有核定位信号（NLS），导致FGF12在细胞核中积累而没有任何细胞释放。FGF12 有两种形式，长形式 (FGF12A) 和短形式 (FGF12B)。FGF12B缺乏NLS，因为FGF12A的N端66个氨基酸残基通过选择性剪接被4个氨基酸取代。FGF12 可通过质膜转位至细胞内而在组织中发挥作用，这种新型 CPP 的出现为生物活性分子的细胞内递送提供了新工具^[1]。

1. The ED₅₀ is <20 ng/mL as measured by TF-1 cells, corresponding to a specific activity of >5 × 10⁴ units/mg.
2. Immobilized Human FGF-12 at 2.0 μg/ml (100 μl/well) can bind Human FGFR3-Fc. The ED₅₀ of Human FGF-12 is 0.5-4.0 μg/ml.
3. Measured in a cell proliferation assay using NIH-3T3 mouse fibroblast cells. The ED₅₀ this effect is 9.139 ng/mL, corresponding to a specific activity is 1.094×10^5 U/mg.

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  easured in a cell proliferation assay using NIH-3T3 mouse fibroblast cells. The ED₅₀ for this effect is 9.139 ng/mL, corresponding to a specific activity is 1.094×10⁵ U/mg.

Human

E. coli

Tag Free

P61328-1 (E67-T243)

2257  [NCBI]

MESKEPQLKGIVTRLFSQQGYFLQMHPDGTIDGTKDENSDYTLFNLIPVGLRVVAIQGVKASLYVAMNGEGYLYSSDVFTPECKFKESVFENYYVIYSSTLYRQQESGRAWFLGLNKEGQIMKGNRVKKTKPSSHFVPKPIEVCMYREPSLHEIGEKQGRSRKSSGTPTMNGGKVVNQDST

Approximately 20.4 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Nakayama F, et al. Fibroblast growth factor-12 (FGF12) translocation into intestinal epithelial cells is dependent on a novel cell-penetrating peptide domain: involvement of internalization in the in vivo role of exogenous FGF12. J Biol Chem. 2011 Jul 22;286(29):25823-34.  [Content Brief]