FGF-2 蛋白, Mouse (154a.a)

FGF-2 protein is a member of the fibroblast growth factor family, involved in biological processes such as bone healing, cartilage repair, tumor development, and nerve regeneration. FGF-2 is also a mitogen that accelerates cell proliferation. It regulates immune processes by specifically targeting tyrosine kinase receptors and activating the FGF/FGFR signaling pathway. FGF-2 protein, Mouse (154 a.a.), is a recombinant protein produced in E. coli (Escherichia coli), consisting of 154 amino acids (M1-S154), and is untagged^{[1][2][3][4][5][6][7][8][9]}.

FGF-2 蛋白属于与肝素结合 FGF 生长因子家族，FGF-2 蛋白在肌腱至骨愈合、软骨修复、骨修复、神经再生及癌症发生等过程中发挥重要作用^[1][4]。
(1) 骨细胞增殖：低剂量 FGF-2 蛋白可刺激骨髓间充质干细胞的增殖和分化，并上调I/III型胶原和纤连蛋白的 mRNA 表达。然而，高剂量的 FGF-2 蛋白不会刺激细胞外基质 (ECM) 蛋白增殖和基因表达。FGF-2 蛋白也是软骨修复中的内源性和内在生长因子。FGF-2 蛋白与硫酸乙酰肝素蛋白多糖结合并储存在关节软骨的 ECM 中。当软骨受损或退化时，ECM 迅速释放 FGF-2 并激活 ERK 信号通路，促进软骨再生。FGF-2 与其特定受体结合表现出双相效应。FGF-2 与 FGFR3 联合促进关节软骨的修复。FGF-2 与 FGFR1 联合促进关节软骨退化^[1]。
(2) 诱导癌症发生：FGF-2 可增强胶质母细胞瘤干细胞的自我更新，有助于胶质瘤的生长和血管化^[2]。FGF-2 信号转导失调会诱导癌症发生，FGF-2 蛋白是肿瘤微环境中的关键肿瘤促进因子^[6]。
(3) 神经发生：FGF-2 蛋白是大脑皮层细胞增殖和神经发生所必须的生长因子。^[3] FGF-2 蛋白通过 β-连环蛋白介导来调控神经干细胞的增殖和神经元分化。在 FGF-2 蛋白存在下，β-连环蛋白的过表达有助于维持神经祖细胞处于增殖状态。然而，在缺乏 FGF-2 蛋白的情况下，β-连环蛋白的过表达会增强神经元分化^[8]。
(4) 诱导炎症：FGF-2 蛋白水平升高会导致血管生成和炎症反应失调，可诱导多种炎症相关基因的表达，包括促炎细胞因子/趋化因子及其受体、内皮细胞粘附分子和前列腺素通路成分^[4]。
(5) 血管生成：FGF-2 蛋白通过与各种内皮细胞表面受体相互作用发挥促血管生成活性，包括酪氨酸激酶受体、硫酸半肝素蛋白聚糖和整合素^[4]。体内 FGF-2 蛋白过表达会导致肺动脉平滑肌细胞 (SMC) 增生，体内 FGF2 蛋白含量降低可有效预防肺动脉高压^[7]。
FGF-2 蛋白在某些物种中高度保守，人 FGF-2 蛋白序列与大鼠、小鼠和牛的相似性分别为97.4%、95.45%和98.71%。

FGF-2 蛋白 (Mouse) (0-50 ng/mL，30 分钟 - 2 小时) 以剂量依赖性方式上调 CD1 小鼠神经祖细胞中 β-连环蛋白 mRNA 的水平，从而调控神经细胞的增殖分化^[8]。

FGF-2 蛋白 (Mouse) (2 和 6 μg/kg，静脉注射，单次给药 ) 可显著增强约克夏猪慢性缺血模型 (通过手术构建) 的心肌功能^[9]。
FGF-2 蛋白 (Mouse) (5 ng/g，腹腔注射，每日一次共 21 天) 可减少高焦虑属性 (LR) Sprague Dawley 大鼠 (通过选择性繁育获得) 的焦虑样行为以及提高其海马体细胞存活率^[5]。

Measured in a cell proliferation assay using BALB/c 3T3 cells. The ED₅₀ for this effect is 0.3-2.205 ng/mL.

• 
  Measured in a cell proliferation assay using Balb/3T3 cells. The ED₅₀ for this effect is 2.205 ng/mL, corresponding to a specific activity is 4.52×10⁵ units/mg.

Mouse

E. coli

Tag Free

P15655 (M1-S154)

14173  [NCBI]

MAASGITSLPALPEDGGAAFPPGHFKDPKRLYCKNGGFFLRIHPDGRVDGVREKSDPHVKLQLQAEERGVVSIKGVCANRYLAMKEDGRLLASKCVTEECFFFERLESNNYNTYRSRKYSSWYVALKRTGQYKLGSKTGPGQKAILFLPMSAKS

Approximately 17 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 400 mM NaCl, 0.02% Tween 80, 4.0% Sucrose, 4.0% Manntiol, pH 7.0 or PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Zhang J, et al. FGF2: a key regulator augmenting tendon-to-bone healing and cartilage repair. Regen Med. 2020 Sep;15(9):2129-2142.  [Content Brief]

  [2]. Jimenez-Pascual A, et al. FGF2: a novel druggable target for glioblastoma? Expert Opin Ther Targets. 2020 Apr;24(4):311-318.  [Content Brief]

  [3]. Raballo R, et al. Basic fibroblast growth factor (Fgf2) is necessary for cell proliferation and neurogenesis in the developing cerebral cortex[J]. Journal of Neuroscience, 2000, 20(13): 5012-5023.  [Content Brief]

  [4]. Presta M, et al. Inflammatory cells and chemokines sustain FGF2-induced angiogenesis. Eur Cytokine Netw. 2009 Jun;20(2):39-50.  [Content Brief]

  [5]. Perez JA, et al. A new role for FGF2 as an endogenous inhibitor of anxiety. J Neurosci. 2009 May 13;29(19):6379-87.  [Content Brief]

  [6]. Akl MR, et al. Molecular and clinical significance of fibroblast growth factor 2 (FGF2 /bFGF) in malignancies of solid and hematological cancers for personalized therapies. Oncotarget. 2016 Jul 12;7(28):44735-44762.  [Content Brief]

  [7]. Izikki M, et al. Endothelial-derived FGF2 contributes to the progression of pulmonary hypertension in humans and rodents. J Clin Invest. 2009 Mar;119(3):512-23.  [Content Brief]

  [8]. Israsena N, et al. The presence of FGF2 signaling determines whether beta-catenin exerts effects on proliferation or neuronal differentiation of neural stem cells. Dev Biol. 2004 Apr 1;268(1):220-31.  [Content Brief]

  [9]. Sato K, et al. Efficacy of intracoronary versus intravenous FGF-2 in a pig model of chronic myocardial ischemia. Ann Thorac Surg. 2000 Dec;70(6):2113-8.  [Content Brief]