1. 重组蛋白
  2. Cytokines and Growth Factors Biotinylated Proteins
  3. FGF Family
  4. Fibroblast Growth Factor
  5. Fibroblast Growth Factor 21 (FGF-21)
  6. FGF-21 蛋白, Human (Biotinylated, HEK293, mFc-Avi)

FGF-21 蛋白, Human (Biotinylated, HEK293, mFc-Avi)

目录号: HY-P700719
产品使用指南

FGF-21 蛋白在促进脂肪细胞葡萄糖摄取方面发挥关键作用,可诱导 SLC2A1/GLUT1 表达,但不影响 SLC2A4/GLUT4,具体取决于 KLB。它显着调节全身葡萄糖稳态和胰岛素敏感性,并通过其 C 末端与 KLB 直接相互作用。与 FGFR4 的结合增加了其分子机制的复杂性,突出了其在协调局部效应之外的细胞反应中的作用。FGF-21 蛋白, Human (Biotinylated, HEK293, mFc-Avi) 是重组的 FGF-21 蛋白,由 HEK293 表达,带有 N-Avi, N-mFc 标签。

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描述

FGF-21 Proteinas, pivotal in promoting glucose uptake in adipocytes, induces SLC2A1/GLUT1 expression, not affecting SLC2A4/GLUT4, contingent on KLB.It significantly regulates systemic glucose homeostasis and insulin sensitivity, with direct KLB interaction via its C-terminus.Engagement with FGFR4 adds complexity to its molecular mechanisms, highlighting its role in orchestrating cellular responses beyond localized effects.FGF-21 Protein, Human (Biotinylated, HEK293, mFc-Avi) is the recombinant human-derived FGF-21 protein, expressed by HEK293 , with N-Avi, N-mFc labeled tag.

研究背景

FGF-21 蛋白通过特异性诱导葡萄糖转运蛋白 SLC2A1/GLUT1 的表达,同时不影响 SLC2A4/GLUT4 的表达,在促进分化脂肪细胞内的葡萄糖摄取方面发挥关键作用。其活动取决于 KLB 的存在。除了其局部作用外,这种蛋白质还对全身葡萄糖稳态和胰岛素敏感性的调节做出了重大贡献。通过其 C 末端促进与 KLB 的直接相互作用,强调了其功能的分子基础。此外,该蛋白质与 FGFR4 结合,进一步凸显了其在协调细胞反应中相互作用的复杂性。

种属

Human

表达系统

HEK293

标签

N-Avi;N-mFc

蛋白编号

Q9NSA1 (H29-S209)

基因 ID
蛋白结构
N-term
mFc-Avi
FGF-21 (H29-S209)
Accession # Q9NSA1
C-term
同用名
UNQ3115; PRO10196; FGF-21; FGF21
分子量

55-65 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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  • 重组蛋白稀释计算器

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
FGF-21 Protein, Human (Biotinylated, HEK293, mFc-Avi)
目录号:
HY-P700719
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