FGF-23 蛋白, Human (R179Q, HEK293, His)

FGF-23 protein is an important regulator of phosphate homeostasis and inhibits renal tubular phosphate transport by reducing SLC34A1 levels. It upregulates EGR1 expression through KL, directly reduces PTH secretion, and negatively regulates osteoblast differentiation and matrix mineralization. FGF-23 Protein, Human (R179Q, HEK293, His) is the recombinant human-derived FGF-23 protein, expressed by HEK293 , with C-His labeled tag and R179Q, , , , mutation. The total length of FGF-23 Protein, Human (R179Q, HEK293, His) is 227 a.a., with molecular weight of 29-32 kDa.

FGF-23 蛋白是磷酸盐稳态的重要调节剂，其通过降低 SLC34A1 水平抑制肾小管磷酸盐转运的能力证明了这一点。此外，在 KL 存在的情况下，它还能上调 EGR1 的表达，并直接作用于甲状旁腺，减少 PTH 的分泌。该蛋白参与维生素 D 代谢的调节，并作为成骨细胞分化和基质矿化的负调节剂。FGF-23 与 FGFR1、FGFR2、FGFR3 和 FGFR4 的相互作用进一步强调了其在细胞过程中的重要性。此外，KL 和硫酸乙酰肝素糖胺聚糖增强了成纤维细胞生长因子 (FGF) 与其受体之间的亲和力，它们是该调节网络中的关键辅助受体。

Determined by its ability to stimulate the proliferation of NIH-3T3 cells. The ED₅₀ for this effect is 14.84 ng/mL in the presence of 1μg/mL murine Klotho and 100μg/mL heparin, corresponding to a specific activity is 6.739×10⁴ units/mg.

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  Determined by its ability to stimulate the proliferation of NIH-3T3 cells. The ED₅₀ for this effect is 14.84 ng/mL in the presence of 1μg/mL murine Klotho and 100μg/mL heparin, corresponding to a specific activity is 6.739×10⁴ units/mg.

Human

HEK293

C-6*His

Q9GZV9-1 (Y25-I251, R179Q)

8074  [NCBI]

YPNASPLLGSSWGGLIHLYTATARNSYHLQIHKNGHVDGAPHQTIYSALMIRSEDAGFVVITGVMSRRYLCMDFRGNIFGSHYFDPENCRFQHQTLENGYDVYHSPQYHFLVSLGRAKRAFLPGMNPPPYSQFLSRRNEIPLIHFNTPIPRRHTQSAEDDSERDPLNVLKPRARMTPAPASCSQELPSAEDNSPMASDPLGVVRGGRVNTHAGGTGPEGCRPFAKFI

Approximately 29-32 kDa due to the glycosylation.

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.