FGF-23 蛋白, Mouse (HEK293, His)

FGF-23 protein is a key regulator that maintains phosphate homeostasis by inhibiting tubular phosphate transport and reducing SLC34A1 levels. It directly inhibits PTH secretion, regulates vitamin D metabolism, and negatively affects osteoblast differentiation and matrix mineralization. FGF-23 Protein, Mouse (HEK293, His) is the recombinant mouse-derived FGF-23 protein, expressed by HEK293 , with C-6*His labeled tag and R179Q mutation. The total length of FGF-23 Protein, Mouse (HEK293, His) is 227 a.a., with molecular weight of ~30-32 kDa.

FGF-23 蛋白通过抑制肾小管磷酸盐转运（主要是通过降低 SLC34A1 水平）来充当维持磷酸盐稳态的关键调节剂。此外，它还直接作用于甲状旁腺，抑制 PTH 分泌，并在维生素 D 代谢调节中发挥关键作用。FGF-23 充当成骨细胞分化和基质矿化的负调节剂。此外，它表现出在 KL 存在的情况下上调 EGR1 表达的能力，并与各种成纤维细胞生长因子受体 (FGFR) 相互作用，包括 FGFR1、FGFR2、FGFR3 和 FGFR4。KL 和硫酸乙酰肝素糖胺聚糖作为辅助受体促进 FGF-23 和 FGFR 之间的相互作用。

Determined by its ability to stimulate the proliferation of murine NIH-3T3 cells. The ED₅₀ for this effect is 0.2468 μg/mL in the presence of 1 μg/mL murine Klotho and 100μg/mL heparin, corresponding to a specific activity is 4.051×10³units/mg.

Mouse

HEK293

C-6*His

Q9EPC2 (Y25-V251, R179Q)

64654

YPDTSPLLGSNWGSLTHLYTATARTSYHLQIHRDGHVDGTPHQTIYSALMITSEDAGSVVITGAMTRRFLCMDLHGNIFGSLHFSPENCKFRQWTLENGYDVYLSQKHHYLVSLGRAKRIFQPGTNPPPFSQFLARRNEVPLLHFYTVRPRRHTQSAEDPPERDPLNVLKPRPRATPVPVSCSRELPSAEEGGPAASDPLGVLRRGRGDARGGAGGADRCRPFPRFV

Approximately 30-32 kDa due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM MOPS, 150 mM NaCl, pH 7.4, 1 mM EDTA, 1 mM DTT, 8% trehalose.

<0.1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in PBS. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.