1. 重组蛋白
  2. Cytokines and Growth Factors
  3. FGF Family
  4. Fibroblast Growth Factor
  5. FGF-2/bFGF
  6. FGF basic/bFGF 蛋白, Human (P. pastoris, N-His)

FGF basic/bFGF 蛋白, Human (P. pastoris, N-His)

目录号: HY-P700479
COA 产品使用指南

FGF basic 或 bFGF(基本成纤维细胞生长因子)以替代 CUG 密码子起始,标志着翻译起始的独特特征。该替代起始密码子在调节 FGF basic 的表达和功能特性中发挥着关键作用。FGF basic/bFGF 蛋白, Human (P. pastoris, N-His) 是重组的 FGF 碱性/bFGF 蛋白,由 P. pastoris 表达,带有 N-6*His 标签。FGF basic/bFGF 蛋白, Human (P. pastoris, N-His) 全长 146 个氨基酸,分子量为 17.9 kDa。

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规格 价格 是否有货 数量
20 μg ¥1300 In-stock
50 μg ¥2800 In-stock
100 μg ¥4500 In-stock
> 100 μg   询价  

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描述

FGF basic, or bFGF (fibroblast growth factor basic), initiates at an alternative CUG codon, marking a distinctive feature in translational initiation. This alternative start codon plays a pivotal role in regulating the expression and functional properties of FGF basic. FGF basic/bFGF Protein, Human (P. pastoris, N-His) is the recombinant human-derived FGF basic/bFGF protein, expressed by P. pastoris , with N-6*His labeled tag. The total length of FGF basic/bFGF Protein, Human (P. pastoris, N-His) is 146 a.a., with molecular weight of 17.9 kDa.

研究背景

FGF basic(也称为 bFGF(碱性成纤维细胞生长因子))的起始发生在替代 CUG 密码子处。这种替代起始密码子代表了蛋白质翻译起始的独特特征,有助于形成控制 FGF basic 表达和功能特性的独特调节机制。

种属

Human

表达系统

P. pastoris

标签

N-6*His

蛋白编号

P09038-4 (P143-S288)

基因 ID
蛋白结构
N-term
6*His
bFGF (P143-S288)
Accession # P09038-4
C-term
同用名
Fibroblast Growth Factor 2; FGF-2; Basic Fibroblast Growth Factor; bFGF; Heparin-Binding Growth Factor 2; HBGF-2; Fgf2; Fgf-2
氨基酸序列

PALPEDGGSGAFPPGHFKDPKRLYCKNGGFFLRIHPDGRVDGVREKSDPHIKLQLQAEERGVVSIKGVCANRYLAMKEDGRLLASKCVTDECFFFERLESNNYNTYRSRKYTSWYVALKRTGQYKLGSKTGPGQKAILFLPMSAKS

分子量

17.9-20 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0 or PBS, 6% Trehalose, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

FGF basic/bFGF Protein, Human (P. pastoris, N-His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
FGF basic/bFGF Protein, Human (P. pastoris, N-His)
目录号:
HY-P700479
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