FGFR-2 beta (IIIb) 蛋白, Cynomolgus (HEK293, His)

FGFR-2 beta is a tyrosine protein kinase that serves as a cell surface receptor for fibroblast growth factor and is critical for cell regulation, proliferation, differentiation, migration, and apoptosis. It plays important roles in embryonic development, trophoblast function, limb buds, lungs, osteogenesis, and skin development. FGFR-2 beta (IIIb) Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived FGFR-2 beta, expressed by HEK293 , with C-His labeled tag. The total length of FGFR-2 beta (IIIb) Protein, Cynomolgus (HEK293, His) is 215 a.a., with molecular weight of 50-68 kDa.

FGFR-2 beta 是一种酪氨酸蛋白激酶，作为成纤维细胞生长因子的细胞表面受体，在细胞增殖、分化、迁移和凋亡以及胚胎发育的复杂调节中发挥着关键作用。它对于正常胚胎模式、滋养层功能、肢芽发育、肺形态发生、成骨和皮肤发育是不可或缺的。此外，FGFR-2β 对于成骨细胞分化、增殖和凋亡的调节至关重要，对正常骨骼发育有显着贡献。在促进角质形成细胞和未成熟成骨细胞增殖的同时，诱导分化成骨细胞凋亡。通过 PLCG1、FRS2 和 PAK4 的磷酸化，FGFR-2 beta 在配体结合后启动多种信号级联反应。PLCG1 的激活导致二酰基甘油和肌醇 1,4,5-三磷酸的产生。此外，FRS2 的磷酸化会触发 GRB2、GAB1、PIK3R1 和 SOS1 的募集，介导 RAS、MAPK1/ERK2、MAPK3/ERK1、MAP 激酶信号通路和 AKT1 信号通路的激活。FGFR2 信号传导通过泛素化、内化和降解过程受到严格调控。破坏 FGFR-2 正常成熟、内化或降解的突变会导致异常信号传导，包括组成型激酶激活。FGFR-2 的过度表达促进 STAT1 的激活。

Immobilized Cynomolgus FGFR2 beta (IIIb) , His Tag at 0.5 μg/mL (100 μl/Well) on the plate. Dose response curve for Anti-FGFR2 (IIIb) Antibody, hFc Tag with the EC₅₀ of ≤15 ng/mL determined by ELISA.

Cynomolgus

HEK293

C-His

A0A2K5TL84 (P154-K368)

/

50-68 kDa

Greater than 95% as determined by Tris-Bis PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5%-8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.