FGFR-3 蛋白, Human (HEK293, Fc)

The FGFR-3 protein is a tyrosine-protein kinase receptor for fibroblast growth factor that is critical for cellular processes, particularly in chondrocytes, osteoblasts, and inner ear development. Its effects span normal skeletal development and postnatal bone mineralization. FGFR-3 Protein, Human (HEK293, Fc) is the recombinant human-derived FGFR-3 protein, expressed by HEK293 , with C-hFc labeled tag.

FGFR-3蛋白是一种酪氨酸蛋白激酶，作为成纤维细胞生长因子的细胞表面受体，在细胞增殖、分化和凋亡的调节中发挥着重要作用。其在调节软骨细胞分化、增殖和凋亡方面的意义尤其显着，有助于正常的骨骼发育。此外，FGFR-3 在成骨细胞的成骨和出生后骨矿化中发挥着至关重要的作用，同时还促进软骨细胞的凋亡。除了在正常发育中的作用之外，FGFR-3 还参与内耳发育，并对维生素 D 代谢的调节具有影响。配体结合后，FGFR-3 激活多个信号级联反应，包括 PLCG1、CBL 和 FRS2 的磷酸化。这种激活导致细胞信号分子的产生，例如二酰甘油和肌醇 1,4,5-三磷酸。此外，FRS2 的磷酸化会触发 GRB2、GAB1、PIK3R1 和 SOS1 的募集，介导 RAS、MAPK1/ERK2、MAPK3/ERK1、MAP 激酶信号通路和 AKT1 信号通路的激活。导致组成型激酶激活或损害正常 FGFR3 成熟、内化和降解的突变会导致信号传导异常。FGFR3 的过表达或组成型激活可促进 PTPN11/SHP2、STAT1、STAT5A 和 STAT5B 的激活。此外，分泌的异构体 3 保留了结合 FGF1 和 FGF2 的能力，可能干扰 FGF 信号传导。

1.The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.
2.Measured by its ability to inhibit FGF acidic-dependent proliferation of NIH-3T3 mouse fibroblast cells. The ED₅₀ for this effect is 10.64 ng/mL, corresponding to a specific activity is 9.398×10⁴ U/mg.
3.The ED₅₀ as determined by its ability to inhibit FGF acidic-dependent proliferation of BALB/c 3T3 cells is less than 100 ng/mL.

Human

HEK293

C-hFc

P22607-1 (E23-G375)

2261  [NCBI]

ESLGTEQRVVGRAAEVPGPEPGQQEQLVFGSGDAVELSCPPPGGGPMGPTVWVKDGTGLVPSERVLVGPQRLQVLNASHEDSGAYSCRQRLTQRVLCHFSVRVTDAPSSGDDEDGEDEAEDTGVDTGAPYWTRPERMDKKLLAVPAANTVRFRCPAAGNPTPSISWLKNGREFRGEHRIGGIKLRHQQWSLVMESVVPSDRGNYTCVVENKFGSIRQTYTLDVLERSPHRPILQAGLPANQTAVLGSDVEFHCKVYSDAQPHIQWLKHVEVNGSKVGPDGTPYVTVLKTAGANTTDKELEVLSLHNVTFEDAGEYTCLAGNSIGFSHHSAWLVVLPAEEELVEADEAGSVYAG

95-110 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.