FGFR-4 蛋白, Mouse (HEK293, His)

FGFR-4 protein is a tyrosine protein kinase that serves as a cell surface receptor for fibroblast growth factor and controls proliferation, differentiation, migration, lipid metabolism, bile acid biosynthesis, glucose uptake, vitamin D metabolism, and phosphate and other cellular processes homeostasis. It significantly downregulates CYP7A1 in response to FGF19. FGFR-4 Protein, Mouse (HEK293, His) is the recombinant mouse-derived FGFR-4 protein, expressed by HEK293 , with C-His labeled tag. The total length of FGFR-4 Protein, Mouse (HEK293, His) is 196 a.a., with molecular weight of 58-80 kDa.

FGFR-4 蛋白是一种酪氨酸蛋白激酶，作为成纤维细胞生长因子的细胞表面受体，调节细胞增殖、分化、迁移、脂质代谢、胆汁酸生物合成、葡萄糖摄取、维生素 D 代谢和磷酸盐稳态。它对于 CYP7A1 表达的正常下调至关重要，CYP7A1 是参与胆汁酸合成的酶，响应 FGF19。配体结合后，FGFR-4 磷酸化 PLCG1 和 FRS2，导致多个信号级联的激活。PLCG1 的激活导致二酰基甘油和肌醇 1,4,5-三磷酸的产生。FRS2 的磷酸化会招募 GRB2、GAB1、PIK3R1 和 SOS1，从而激活 RAS、MAPK1/ERK2、MAPK3/ERK1 和 AKT1 信号通路。FGFR-4 还促进基质蛋白酶 MMP14 的 SRC 依赖性磷酸化和溶酶体降解，其中 MMP14 有助于 FGFR-4 的内化和降解。此外，FGFR-4 在出生后肺部发育中发挥作用，并可能参与骨骼肌细胞谱系发育。

Measured by its ability to inhibit FGF acidic-dependent proliferation of NIH-3T3 mouse fibroblast cells. The ED₅₀ of this effect is 8.197 ng/ml in the presence of 2 ng/mL FGF-acidic, corresponding to a specific activity is 1.219×10⁵ units/mg.

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  Measured by its ability to inhibit FGF acidic-dependent proliferation of NIH-3T3 mouse fibroblast cells. The ED₅₀ of this effect is 8.197 ng/ml in the presence of 2 ng/mL FGF-acidic, corresponding to a specific activity is 1.219×10⁵ units/mg.

Mouse

HEK293

C-6*His

Q03142/NP_032037.2 (L17-D366)

14186  [NCBI]

LSLEASEEMEQEPCLAPILEQQEQVLTVALGQPVRLCCGRTERGRHWYKEGSRLASAGRVRGWRGRLEIASFLPEDAGRYLCLARGSMTVVHNLTLLMDDSLTSISNDEDPKTLSSSSSGHVYPQQAPYWTHPQRMEKKLHAVPAGNTVKFRCPAAGNPMPTIHWLKDGQAFHGENRIGGIRLRHQHWSLVMESVVPSDRGTYTCLVENSLGSIRYSYLLDVLERSPHRPILQAGLPANTTAVVGSDVELLCKVYSDAQPHIQWLKHVVINGSSFGADGFPYVQVLKTTDINSSEVEVLYLRNVSAEDAGEYTCLAGNSIGLSYQSAWLTVLPEEDLTWTTATPEARYTD

Approximately 58-80 kDa due to the glycosylation.

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.