1. 重组蛋白
  2. Immune Checkpoint Proteins
  3. Inhibitory Checkpoint Molecules
  4. FGL-1
  5. FGL1 蛋白, Mouse (sf9, His)

FGL1 蛋白, Mouse (sf9, His)

目录号: HY-P75188
产品使用指南

FGL1 蛋白是一种有效的免疫抑制分子,作为 LAG3 的主要配体抑制抗原特异性 T 细胞活化。它对 LAG3 介导的 T 细胞抑制功能发挥着至关重要的作用,独立于 MHC II 类。FGL1 由肝细胞分泌,也促进其生长。该蛋白的同二聚体形式与 LAG3 相互作用,通过纤维蛋白原 C 末端结构域特异性地与其 Ig 样结构域 1 和 2 结合。FGL1 蛋白, Mouse (sf9, His) 是重组的 FGL1 蛋白,由 Sf9 insect cells 表达,带有 C-His 标签。

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描述

FGL1 Protein, a potent immune suppressive molecule, inhibits antigen-specific T-cell activation as a major ligand for LAG3. It crucially contributes to LAG3-mediated T-cell inhibitory functions, independently of MHC class II. Secreted by hepatocytes, FGL1 also promotes their growth. The homodimeric form of the protein interacts with LAG3, specifically engaging with its Ig-like domains 1 and 2 through the Fibrinogen C-terminal domain. FGL1 Protein, Mouse (sf9, His) is the recombinant mouse-derived FGL1 protein, expressed by Sf9 insect cells , with C-His labeled tag.

研究背景

FGL1 蛋白是一种有效的免疫抑制分子,作为 LAG3 的主要配体对抗原特异性 T 细胞激活发挥抑制作用。它是 LAG3 介导的 T 细胞抑制功能的重要贡献者,值得注意的是,它与 LAG3 的结合独立于 MHC II 类发生。此外,FGL1由肝细胞分泌,并在促进其生长方面发挥作用。该蛋白以同二聚体形式存在,通过其纤维蛋白原 C 端结构域与 LAG3 相互作用,特别是与 LAG3 的 Ig 样结构域 1 和 2 结合。

种属

Mouse

表达系统

Sf9 insect cells

标签

C-His

蛋白编号

Q71KU9 (L23-I314)

基因 ID
蛋白结构
N-term
FGL1 (L23-I314)
Accession # Q71KU9
His
C-term
同用名
Fibrinogen-like protein 1; FGL1; HP-041; Hepassocin; HFREP-1; LFIRE-1
分子量

Approximately 35.5 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

FGL1 Protein, Mouse (sf9, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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FGL1 Protein, Mouse (sf9, His)
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HY-P75188
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