FIS1 蛋白, Human (His)

The FIS1 protein is essential for mitochondrial network fragmentation and perinuclear clustering. Although FIS1 plays a minor role in recruiting DNM1L to the mitochondrial surface, it is associated with fission. FIS1 Protein, Human (His) is the recombinant human-derived FIS1 protein, expressed by E. coli , with C-6*His labeled tag. The total length of FIS1 Protein, Human (His) is 122 a.a., with molecular weight of ~15.0 kDa.

FIS1 蛋白在线粒体网络的碎片化中发挥着至关重要的作用，有助于其核周聚集。虽然 FIS1 在线粒体表面的裂变介体动力相关蛋白 1 (DNM1L) 募集和结合方面发挥次要作用，但它与线粒体裂变有关。值得注意的是，它对于功能性裂变复合物的组装和随后的膜分裂事件可能不是必需的。除了参与线粒体动力学之外，FIS1 还介导过氧化物酶体裂变，特别是当裂变的结果针对线粒体稳态、线粒体自噬或细胞凋亡时。该蛋白可以诱导细胞色素c从线粒体释放到细胞质中，最终引发细胞凋亡。与 DNM1L/DLP1、MARCHF5、MIEF1 和 PEX11A、PEX11B、PEX11G 的相互作用突出了复杂的关联网络，这些关联网络有助于 FIS1 在线粒体和过氧化物酶体动力学界面上的多方面作用。

Human

E. coli

C-6*His

Q9Y3D6 (M1-G122)

51024  [NCBI]

MEAVLNELVSVEDLLKFEKKFQSEKAAGSVSKSTQFEYAWCLVRSKYNDDIRKGIVLLEELLPKGSKEEQRDYVFYLAVGNYRLKEYEKALKYVRGLLQTEPQNNQAKELERLIDKAMKKDG

Approximately 15.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.