FKBP12 蛋白, Mouse (His)

The FKBP12 protein is multifunctional and critical in TGF-β signaling by maintaining TGFBR1 in an inactive conformation, thereby preventing receptor activation in the absence of ligand. It recruits SMAD7 to ACVR1B, blocks the binding of SMAD2 and SMAD3 to the activin receptor complex, and thereby inhibits activin signaling. FKBP12 Protein, Mouse (His) is the recombinant mouse-derived FKBP12 protein, expressed by E. coli , with N-His labeled tag. The total length of FKBP12 Protein, Mouse (His) is 107 a.a., with molecular weight of ~13 KDa.

FKBP12 是一种多功能蛋白，在维持 TGFBR1（TGF-β 的丝氨酸/苏氨酸激酶受体）的非活性构象方面发挥着至关重要的作用，从而防止在没有配体的情况下受体激活。这种调节功能有助于精确控制 TGF-β 信号传导。此外，FKBP12 将 SMAD7 招募到 ACVR1B，阻碍 SMAD2 和 SMAD3 与激活素受体复合物的结合，并有效阻断激活素信号传导。除了在 TGF-β 和激活素途径中发挥作用之外，FKBP12 通过潜在调节 RYR1 钙通道的活性而表现出多功能性。FKBP12 作为肽基-脯氨酰顺反异构酶 (PPIase)，通过催化寡肽中脯氨酸酰亚胺肽键的顺反异构化来加速蛋白质折叠。这种多样化的功能强调了 FKBP12 在细胞过程中的重要性，并强调了它在各种信号传导途径中的参与。

Measured by its ability to convert the substrate, Suc-AAPF-pNA, from Cis to Trans formation. The specific activity is 7800 pmol/min/μg, as measured under the described conditions.

Mouse

E. coli

N-6*His

P26883 (G2-E108)

14225  [NCBI]

GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKPFKFTLGKQEVIRGWEEGVAQMSVGQRAKLIISSDYAYGATGHPGIIPPHATLVFDVELLKLE

Approximately 13 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.