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  3. FLG 蛋白, Human (P.pastoris, His)

FLG 蛋白, Human (P.pastoris, His)

目录号: HY-P71734
产品使用指南

FLG 蛋白是哺乳动物表皮所必需的,在终末分化中发挥着关键作用。它聚集角蛋白中间丝并促进二硫键形成,强调其在细胞分化的最后阶段对表皮的结构组织和完整性的关键贡献。FLG 蛋白, Human (P.pastoris, His) 是重组的 FLG 蛋白,由 P. pastoris 表达,带有 N-His 标签。FLG 蛋白, Human (P.pastoris, His) 全长 224 个氨基酸,分子量约为 ~26.8 kDa。

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描述

FLG Protein, essential in the mammalian epidermis, plays a key role in terminal differentiation. It aggregates keratin intermediate filaments and promotes disulfide bond formation, emphasizing its crucial contribution to the structural organization and integrity of the epidermis in the concluding stages of cellular differentiation. FLG Protein, Human (P.pastoris, His) is the recombinant human-derived FLG protein, expressed by P. pastoris , with N-His labeled tag. The total length of FLG Protein, Human (P.pastoris, His) is 224 a.a., with molecular weight of ~26.8 kDa.

研究背景

FLG 是哺乳动物表皮中的重要蛋白质,通过聚集角蛋白中间丝并促进这些丝之间二硫键的形成,在终末分化过程中发挥关键作用。这一功能强调了 FLG 在细胞分化最后阶段表皮结构组织和完整性中的重要性。

种属

Human

表达系统

P. pastoris

标签

N-His

蛋白编号

P20930 (3838D-4061E)

基因 ID
蛋白结构
N-term
His
FLG (3838D-4061E)
Accession # P20930
C-term
同用名
ATOD2; Epidermal filaggrin; Filaggrin; Filaggrin precursor; Fillagrin; FLG; Profilaggrin
氨基酸序列

DSSRHSQSGQGESAGSRRSRRQGSSVSQDSDSEAYPEDSERRSESASRNHHGSSREQSRDGSRHPGSSHRDTASHVQSSPVQSDSSTAKEHGHFSSLSQDSAYHSGIQSRGSPHSSSSYHYQSEGTERQKGQSGLVWRHGSYGSADYDYGESGFRHSQHGSVSYNSNPVVFKERSDICKASAFGKDHPRYYATYINKDPGLCGHSSDISKQLGFSQSQRYYYYE

分子量

Approximately 26.8 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

FLG Protein, Human (P.pastoris, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
FLG Protein, Human (P.pastoris, His)
目录号:
HY-P71734
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