FLRT2 蛋白, Mouse (HEK293, His)

The FLRT2 protein plays a key role in cell-cell adhesion, migration, and axonal guidance. Its interaction with ADGRL3 promotes cell-cell adhesion and promotes cortical neuronal migration through interaction with UNC5D. FLRT2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived FLRT2 protein, expressed by HEK293 , with C-His labeled tag. The total length of FLRT2 Protein, Mouse (HEK293, His) is 504 a.a., with molecular weight of 60-80 kDa.

FLRT2 蛋白在多种细胞过程中发挥着至关重要的作用，包括细胞间粘附、细胞迁移和轴突引导。它与 ADGRL3 以及邻近细胞上表达的其他潜在的懒虫蛋白相互作用，促进细胞间粘附，同时还通过与 UNC5D 相互作用，在大脑发育过程中的皮质神经元迁移中发挥作用。在轴突引导中，FLRT2 介导生长锥塌陷并对神经元引导表现出排斥作用，与 UNC5D 和潜在的其他 UNC-5 家族成员相互作用。此外，FLRT2 还参与成纤维细胞生长因子介导的信号级联反应。FLRT2 对于胚胎发生过程中正常的心脏基底膜组织至关重要，也是适当的胚胎心外膜和心脏形态发生所必需的。通过富含亮氨酸重复序列的自缔合，FLRT2 形成同源寡聚物并与各种伙伴相互作用，包括 FGFR1、FGFR2、ADGRL1/LPHN1、ADGRL3、UNC5D、UNC5B 和 FN1。

Measured by the ability of the immobilized protein to support the adhesion of Neuro-2A mouse neuroblastoma cells. The ED₅₀ for this effect is 0.8205 µg/mL, corresponding to a specific activity is 1.219×10³ units/mg.

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  Measured by the ability of the immobilized protein to support the adhesion of Neuro-2A mouse neuroblastoma cells. The ED₅₀ for this effect is 0.8205 µg/mL, corresponding to a specific activity is 1.219×10³ units/mg.

Mouse

HEK293

C-10*His

Q8BLU0 (C36-S539)

399558  [NCBI]

CPSVCRCDRNFVYCNERSLTSVPLGIPEGVTVLYLHNNQINNAGFPAELHNVQSVHTVYLYGNQLDEFPMNLPKNVRVLHLQENNIQTISRAALAQLLKLEELHLDDNSISTVGVEDGAFREAISLKLLFLSKNHLSSVPVGLPVDLQELRVDENRIAVISDMAFQNLTSLERLIVDGNLLTNKGIAEGTFSHLTKLKEFSIVRNSLSHPPPDLPGTHLIRLYLQDNQINHIPLTAFANLRKLERLDISNNQLRMLTQGVFDHLSNLKQLTARNNPWFCDCSIKWVTEWLKYIPSSLNVRGFMCQGPEQVRGMAVRELNMNLLSCPTTTPGLPVFTPAPSTVSPTTQSPTLSVPSPSRGSVPPAPTPSKLPTIPDWDGRERVTPPISERIQLSIHFVNDTSIQVSWLSLFTVMAYKLTWVKMGHSLVGGIVQERIVSGEKQHLSLVNLEPRSTYRICLVPLDAFNYRTVEDTICSEATTHASYLNNGSNTASSHEQTTSHSMGS

Approximately 60-80 kDa due to the glycosylation.

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.