FLT3 蛋白, Human (T227M, HEK293, His)

FLT3 is a tyrosine-protein kinase receptor that regulates differentiation, proliferation, and survival of hematopoietic progenitor cells and dendritic cells. It phosphorylates downstream effectors such as SHC1 and AKT1, activating signaling cascades involving MTOR, RAS, and MAP kinases. FLT3 Protein, Human (T227M, HEK293, His) is the recombinant human-derived FLT3 protein, expressed by HEK293 , with C-His labeled tag.

FLT3 是一种酪氨酸蛋白激酶，作为细胞因子 FLT3LG 的细胞表面受体发挥作用，对造血祖细胞和树突状细胞的分化、增殖和存活进行调节控制。该受体促进各种下游效应子的磷酸化，包括 SHC1 和 AKT1，并激活涉及 MTOR、RAS、MAPK1/ERK2 和/或 MAPK3/ERK1 的信号级联。此外，它在 FES、FER、PTPN6/SHP、PTPN11/SHP-2、PLCG1 以及 STAT5A 和/或 STAT5B 的磷酸化中发挥关键作用。虽然野生型 FLT3 激活会导致 STAT5A 或 STAT5B 适度激活，但引起组成型激酶活性的突变会导致细胞增殖增强和细胞凋亡抵抗力增强，从而强调了其在促进异常信号传导途径中的作用。

Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human Flt-3 Ligand at 10 μg/mL can bind Human Flt-3. The ED₅₀ for this effect is 0.3422 μg/mL.

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  Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human Flt-3 Ligand at 10 μg/mL can bind Human Flt-3 .The ED₅₀ for this effect is 0.3422 μg/mL.

Human

HEK293

C-10*His

P36888-1 (N27-N541, T227M)

2322  [NCBI]

NQDLPVIKCVLINHKNNDSSVGKSSSYPMVSESPEDLGCALRPQSSGTVYEAAAVEVDVSASITLQVLVDAPGNISCLWVFKHSSLNCQPHFDLQNRGVVSMVILKMTETQAGEYLLFIQSEATNYTILFTVSIRNTLLYTLRRPYFRKMENQDALVCISESVPEPIVEWVLCDSQGESCKEESPAVVKKEEKVLHELFGMDIRCCARNELGRECTRLFTIDLNQTPQTTLPQLFLKVGEPLWIRCKAVHVNHGFGLTWELENKALEEGNYFEMSTYSTNRTMIRILFAFVSSVARNDTGYYTCSSSKHPSQSALVTIVEKGFINATNSSEDYEIDQYEEFCFSVRFKAYPQIRCTWTFSRKSFPCEQKGLDNGYSISKFCNHKHQPGEYIFHAENDDAQFTKMFTLNIRRKPQVLAEASASQASCFSDGYPLPSWTWKKCSDKSPNCTEEITEGVWNRKANRKVFGQWVSSSTLNMSEAIKGFLVKCCAYNSLGTSCETILLNSPGPFPFIQDN

Approximately 80-110 kDa due to the glycosylation.

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.