1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Follistatin-like 1/FSTL1 蛋白, Rat (HEK293, C-His)

Follistatin-like 1/FSTL1 蛋白, Rat (HEK293, C-His)

目录号: HY-P75781A
COA 产品使用指南

卵泡抑素样 1/FSTL1 蛋白结合钙离子和肝素。它参与内皮细胞分化、迁移和 BMP 信号传导调节。FSTL1 还有助于造血干细胞稳态。它位于细胞外区域,在心脏和肺等组织中表达。基因组资源联盟支持其职能。Follistatin-like 1/FSTL1 蛋白, Rat (HEK293, C-His) 是重组的 卵泡抑素样 FSTL1 蛋白,由 HEK293 表达,带有 C-10*His 标签。Follistatin-like 1/FSTL1 蛋白, Rat (HEK293, C-His) 全长 288 个氨基酸,分子量为 37-48 kDa。

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规格 价格 是否有货 数量
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2 μg ¥350 In-stock
10 μg ¥630 In-stock
50 μg ¥1760 In-stock
100 μg ¥3000 In-stock
500 μg ¥8400 In-stock
1 mg ¥14280 询价
> 1 mg   询价  

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

The Follistatin-like 1/FSTL1 protein binds calcium ions and heparin. It is involved in endothelial cell differentiation, migration, and BMP signaling regulation. FSTL1 also contributes to hematopoietic stem cell homeostasis. It is located in the extracellular region and is expressed in tissues like the heart and lung. The Alliance of Genome Resources supports its function. Follistatin-like 1/FSTL1 Protein, Rat (HEK293, C-His) is the recombinant rat-derived Follistatin-like 1/FSTL1 protein, expressed by HEK293 , with C-10*His labeled tag. The total length of Follistatin-like 1/FSTL1 Protein, Rat (HEK293, C-His) is 288 a.a., with molecular weight of 37-48 kDa.

研究背景

卵泡抑素样 1 (FSTL1) 蛋白预计具有钙离子结合和肝素结合活性。预计它还参与内皮细胞分化、内皮细胞迁移和 BMP 信号通路调节等过程。此外,预计它在造血干细胞稳态中发挥作用,并且位于细胞外区域。FSTL1 蛋白与人类 FSTL1 基因同源,其功能得到基因组资源联盟的支持。它在心脏、肺和其他九种组织等组织中表现出偏向表达。

生物活性

Immobilized Recombinant Follistatin-like 1/FSTL1 at 2 μg/ml (100 μl/well) can bind Biotinylated Recombinant human BMP-4 Protein. The ED50 for this effect is 101.5 ng/mL.

  • Immobilized Recombinant Follistatin‑like 1/FSTL1 at 2 μg/ml (100 μl/well) can bind Biotinylated Recombinant human BMP-4 Protein. The ED50 for this effect is 101.5 ng/mL.
种属

Rat

表达系统

HEK293

标签

C-10*His

蛋白编号

NP_077345.1 (E19-I306)

基因 ID

79210

蛋白结构
N-term
FSTL1 (E19-I306)
Accession # NP_077345.1
10*His
C-term
同用名
Follistatin-related protein 1; Follistatin-like protein 1; FSTL1; FRP
氨基酸序列

EEEQRSKSKICANVFCGAGRECAVTEKGEPTCLCIEQCKPHKRPVCGSNGKTYLNHCELHRDACLTGSKIQVDYDGHCKEKKSVSPSASPVVCYQANRDELRRRIIQWLEAEIIPDGWFSKGSNYSEILDKYFKSFDNGDSHLDSSEFLKFVEQNETAVNITAYPNQENNKLLRGLCVDALIELSDENADWKLSFQEFLKCLNPSFNPPEKKCALEDETYADGAETEVDCNRCVCSCGHWVCTAMTCDGKNQKGVQTHTEEEMTRYAQELQKHQGTAEKTKKVNTKEI

分子量

37-48 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

Follistatin-like 1/FSTL1 Protein, Rat (HEK293, C-His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Follistatin-like 1/FSTL1 Protein, Rat (HEK293, C-His)
目录号:
HY-P75781A
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