Fractalkine/CX3CL1 蛋白, Human

Fractalkine/CX3CL1 Protein, Human is found to be associated with inflammatory diseases such as rheumatoid arthritis (RA), rheumatoid vasculitis (RV).

Fractalkine/CX3CL1 以两种形式存在：作为膜锚定的或作为脱落的 80-95K 糖蛋白。Fractalkine/CX3CL1 在炎症条件下在单核细胞中表达。Fractalkine/CX3CL1 还在类风湿性关节炎 (RA) 滑膜组织的巨噬细胞、成纤维细胞、内皮细胞和树突细胞中表达。CX3CL1 与不同疾病的发生相关，例如类风湿性关节炎 (RA)、类风湿性血管炎 (RV)、干燥综合征 (SS)、系统性红斑狼疮 (SLE)、硬皮病、多发性硬化症和动脉粥样硬化^{[1]< /sup>。}

1.Full biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration of 5.0-10 ng/mL.
2.Measured by its ability to chemoattract THP-1 cells. The ED50 for this effect is 7.841 ng/mL, corresponding to a specific activity is 1.275×10⁵ U/mg.

• 
  Measured by its ability to chemoattract THP-1 cells. The ED₅₀ for this effect is 7.841 ng/mL, corresponding to a specific activity is 1.275×10⁵ U/mg.

Human

E. coli

Tag Free

P78423 (Q25-G100)

6376  [NCBI]

QHHGVTKCNITCSKMTSKIPVALLIHYQQNQASCGKRAIILETRQHRLFCADPKEQWVKDAMQHLDRQAAALTRNG

Approximately 8.6 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized after extensive dialysis against 20 mM PB, pH 7.4, 50 mM NaCl or PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Jones BA, et al. Fractalkine/CX3CL1: a potential new target for inflammatory diseases. Mol Interv. 2010 Oct;10(5):263-70.  [Content Brief]