Fractalkine/CX3CL1 蛋白, Rat

Fractalkine/CX3CL1 protein is a versatile chemokine that acts as a ligand for CX3CR1 and integrins ITGAV:ITGB3 and ITGA4:ITGB1. It regulates immune response, inflammation, adhesion, and chemotaxis. It activates integrins via CX3CR1-dependent and -independent pathways, binding to site 1 with CX3CR1 and site 2 without CX3CR1. Soluble Fractalkine/CX3CL1 attracts T-cells and monocytes, not neutrophils. Fractalkine/CX3CL1 Protein, Rat is the recombinant rat-derived Fractalkine/CX3CL1 protein, expressed by E. coli , with tag free. The total length of Fractalkine/CX3CL1 Protein, Rat is 79 a.a., with molecular weight of ~11 kDa.

Fractalkine/CX3CL1 蛋白是一种多面趋化因子，充当 CX3CR1 和整合素 ITGAV:ITGB3 和 ITGA4:ITGB1 的配体。CX3CR1-CX3CL1 信号级联在不同的组织区室中表现出多种功能，包括免疫反应调节、炎症调节、细胞粘附和趋化性中的作用。具体来说，它在内皮细胞的白细胞粘附和迁移过程的调节中起着至关重要的作用。Fractalkine/CX3CL1 表现出以 CX3CR1 依赖性和 CX3CR1 独立方式激活整合素的能力。当 CX3CR1 存在时，它通过与整合素中的经典配体结合位点（位点 1）结合来激活整合素。相反，在缺乏 CX3CR1 的情况下，它会与整合素上的不同位点（位点 2）结合，从而增强其他整合素配体与位点 1 的结合。尽管如此，Fractalkine/CX3CL1 的可溶形式对 T 细胞和单核细胞表现出趋化特性不适用于中性粒细胞。

Measured by its ability to chemoattract THP-1 human acute monocytic leukemia cells. The ED₅₀ for this effect is 5.736 ng/mL, corresponding to a specific activity is 1.74×10^5 U/mg.

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  Measured by its ability to chemoattract THP-1 human acute monocytic leukemia cells.The ED₅₀ this effect is 5.736 ng/mL,corresponding to a specific activity is 1.74×10⁵ U/mg.

Rat

E. coli

Tag Free

O55145 (L22-G100)

89808  [NCBI]

LAGQHLGMTKCNITCHKMTSPIPVTLLIHYQLNQESCGKRAIILETRQHRHFCADPKEKWVQDAMKHLDHQTAALTRNG

Approximately 11 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.8.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.