Fumarate hydratase/FH 蛋白, Human

The fumarate hydratase/FH protein plays a key role in cellular processes as it catalyzes the hydration of fumarate to L-malate in the tricarboxylic acid (TCA) cycle, which is essential for energy production through the production of NADH . Fumarate hydratase/FH Protein, Human is the recombinant human-derived Fumarate hydratase/FH protein, expressed by E. coli , with tag free.

富马酸水合酶 (FH) 是一种催化富马酸可逆转化为 L-苹果酸的酶。这种立体特异性互变在三羧酸（TCA）循环中发挥着关键作用，三羧酸循环是参与能量产生的中心代谢途径。具体来说，FH 促进富马酸水合为 L-苹果酸，有助于 TCA 循环中的关键过渡步骤。在不同物种中进行的实验表明，FH 的特定亚型可能在确定的途径中发挥作用，并且表现出对一个反应方向优于另一个反应方向的偏好。通过参与 TCA 循环，FH 确保以 NADH 形式有效产生能量，凸显其在细胞代谢中的重要作用。

Measured by its ability to catalyze the conversion of fumarate to malic acid. Defines one unit of enzyme activity as 1 nmmol NADH per minute of protein produced as one unit of enzyme activity. The specific activity is 157.155 U/mg

Human

E. coli

Tag Free

P07954-1 (A45-K510)

2271  [NCBI]

ASQNSFRIEYDTFGELKVPNDKYYGAQTVRSTMNFKIGGVTERMPTPVIKAFGILKRAAAEVNQDYGLDPKIANAIMKAADEVAEGKLNDHFPLVVWQTGSGTQTNMNVNEVISNRAIEMLGGELGSKIPVHPNDHVNKSQSSNDTFPTAMHIAAAIEVHEVLLPGLQKLHDALDAKSKEFAQIIKIGRTHTQDAVPLTLGQEFSGYVQQVKYAMTRIKAAMPRIYELAAGGTAVGTGLNTRIGFAEKVAAKVAALTGLPFVTAPNKFEALAAHDALVELSGAMNTTACSLMKIANDIRFLGSGPRSGLGELILPENEPGSSIMPGKVNPTQCEAMTMVAAQVMGNHVAVTVGGSNGHFELNVFKPMMIKNVLHSARLLGDASVSFTENCVVGIQANTERINKLMNESLMLVTALNPHIGYDKAAKIAKTAHKNGSTLKETAIELGYLTAEQFDEWVKPKDMLGPK

Approximately 47.83 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filtered solution of 20 mM Tris, pH 8.0.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.