1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. FUT8 蛋白, Hamster (sf9, His)

FUT8 蛋白, Hamster (sf9, His)

目录号: HY-P75783
COA 产品使用指南

FUT8 蛋白催化岩藻糖添加到 N-聚糖中的初始 GlcNAc 残基上。FUT8 蛋白, Hamster (sf9, His) 是重组的 FUT8 蛋白,由 Sf9 insect cells 表达,带有 C-His 标签。FUT8 蛋白, Hamster (sf9, His) 全长 508 个氨基酸,分子量约为 ~55 kDa。

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规格 价格 是否有货 数量
50 μg ¥8500 In-stock
100 μg   询价  

* Please select Quantity before adding items.

FUT8 Protein, Hamster (sf9, His) 相关产品:

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

FUT8 protein catalyzes the addition of fucose to the initial GlcNAc residue in N-glycans. FUT8 Protein, Hamster (sf9, His) is the recombinant FUT8 protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of FUT8 Protein, Hamster (sf9, His) is 508 a.a., with molecular weight of ~55 kDa.

研究背景

FUT8 蛋白充当一种酶,负责催化 α1-6 连接中的岩藻糖添加到与 N-聚糖中的肽链相邻的初始 GlcNAc 残基上。

生物活性

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

种属

Others

表达系统

Sf9 insect cells

标签

C-His

蛋白编号

G3HCE4 (R68-K575)

基因 ID

100751648  [NCBI]

蛋白结构
N-term
FUT8 (R68-K575)
Accession # G3HCE4
His
C-term
同用名
Alpha-(1,6)-fucosyltransferase; Alpha1-6FucT; FUT8; Fucosyltransferase 8
分子量

Approximately 55 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE

性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, 3 mM DTT, 10% Glycerol, pH 7.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

FUT8 Protein, Hamster (sf9, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
FUT8 Protein, Hamster (sf9, His)
目录号:
HY-P75783
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