FZD3 蛋白, Human (Cell-Free, His)

FZD3 is a Wnt receptor that primarily activates the β-catenin canonical pathway, involving disheveled protein, GSK-3 kinase inhibition, nuclear β-catenin accumulation, and Wnt target gene induction. FZD3 Protein, Human (Cell-Free, His) is the recombinant human-derived FZD3 protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of FZD3 Protein, Human (Cell-Free, His) is 644 a.a., with molecular weight of 75.3 kDa.

FZD3 作为 Wnt 蛋白的受体，主要参与 β-catenin 经典信号通路，协调蓬乱蛋白的激活、GSK-3 激酶的抑制、β-catenin 的核积累以及 Wnt 靶基因的诱导。鉴于 PKC 在 Wnt 介导的 GSK-3 激酶失活中的关键作用，在某些家族成员中观察到的涉及 PKC 和钙通量的次级信号传导途径引发了关于其独特性质以及与经典途径的潜在整合的问题。与 G 蛋白的相互作用是这两条途径的组成部分。Wnt5A 激活通过 G 蛋白依赖性机制刺激 PKC 活性。FZD3 参与组织形态发生和组织分化过程中极性信息的转导，在控制早期轴突生长和引导过程中发挥着关键作用。它对于中枢神经系统主要纤维束的发育至关重要，并调节特定脑和脊髓运动神经元群的轴突生长。此外，FZD3 参与颅神经嵴细胞的迁移并有助于感觉信息的传递。它在神经管闭合中与 FZD6 合作，并参与建立平面细胞极性，特别是在听觉和前庭感觉细胞中定向静纤毛束。此外，FZD3 通过β-连环蛋白依赖性机制维持细胞周期内的交感神经母细胞，从而促进神经发生。与 VANGL2 的相互作用进一步强调了它在细胞过程中的多方面作用。

Human

E. coli Cell-free

N-10*His

Q9NPG1 (H23-A666)

7976

HSLFSCEPITLRMCQDLPYNTTFMPNLLNHYDQQTAALAMEPFHPMVNLDCSRDFRPFLCALYAPICMEYGRVTLPCRRLCQRAYSECSKLMEMFGVPWPEDMECSRFPDCDEPYPRLVDLNLAGEPTEGAPVAVQRDYGFWCPRELKIDPDLGYSFLHVRDCSPPCPNMYFRREELSFARYFIGLISIICLSATLFTFLTFLIDVTRFRYPERPIIFYAVCYMMVSLIFFIGFLLEDRVACNASIPAQYKASTVTQGSHNKACTMLFMILYFFTMAGSVWWVILTITWFLAAVPKWGSEAIEKKALLFHASAWGIPGTLTIILLAMNKIEGDNISGVCFVGLYDVDALRYFVLAPLCLYVVVGVSLLLAGIISLNRVRIEIPLEKENQDKLVKFMIRIGVFSILYLVPLLVVIGCYFYEQAYRGIWETTWIQERCREYHIPCPYQVTQMSRPDLILFLMKYLMALIVGIPSVFWVGSKKTCFEWASFFHGRRKKEIVNESRQVLQEPDFAQSLLRDPNTPIIRKSRGTSTQGTSTHASSTQLAMVDDQRSKAGSIHSKVSSYHGSLHRSRDGRYTPCSYRGMEERLPHGSMSRLTDHSRHSSSHRLNEQSRHSSIRDLSNNPMTHITHGTSMNRVIEEDGTSA

75.3 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of PBS, 0.05% Brij-78, 6%Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add 5-50% of glycerol (final concentration). Our default final concentration of glycerol is 50%. Customers could use it as reference.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.