G-CSF 蛋白, Human (CHO)

G-CSF Protein, Human (CHO) is a polypeptide growth factor that regulates the production of neutrophilic granulocytes.

重组人粒细胞集落刺激因子（CHO 表达）是一种多肽生长因子，可调节中性粒细胞的产生。粒细胞集落刺激因子 (G-CSF) 是一种细胞因子，通常在骨髓微环境中发挥作用，刺激血细胞形成。它选择性地促进中性粒细胞祖细胞的生长和成熟。粒细胞集落刺激因子受体存在于骨髓中的前体细胞上。通过与这些受体结合，粒细胞集落刺激因子启动增殖和分化为成熟粒细胞，并刺激骨髓细胞释放到循环中。除促进生长外，粒细胞集落刺激因子还影响中性粒细胞和单核细胞的吞噬作用、运动性、杀菌活性和表面分子表达^[1][2]。G-CSF 的产生是由几种炎症刺激物诱导的，这些炎症刺激物在感染期间迅速升高，例如白细胞介素-1β (IL-1β)、肿瘤坏死因子-α (TNFα) 和脂多糖 (LPS)。因此，病原体通过 LPS 介导的宿主模式识别受体激活以及宿主细胞因子对感染的反应，可诱导 G-CSF^[3] 的循环水平。

Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED₅₀ for this effect is <100 pg/mL, corresponding to a specific activity is > 1×10⁷ units/mg.

• 
  Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED₅₀ for this effect is 10.95 pg/ml, corresponding to a specific activity is 1×10⁷ units/mg.

Human

CHO

Tag Free

Q8N4W3 (T27-P200)

1440  [NCBI]

TPLGPASSLPQSFLLKCLEQVRKIQGDGAALQEKLCATYKLCHPEELVLLGHSLGIPWAPLSSCPSQALQLAGCLSQLHSGLFLYQGLLQALEGISPELGPTLDTLQLDVADFATTIWQQMEELGMAPALQPTQGAMPAFASAFQRRAGGVLVASHLQSFLEVSYRVLRHLAQP

Approximately 18.3 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS or 20 mM PB, 150 mM NaCl, pH 7.4 or PBS, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween80.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Demetri GD, et al. Granulocyte Colony Stimulating Factor and its receptor. Blood. 1991 Dec 1;78(11):2791-808.  [Content Brief]

  [2]. Shah J, et al. The clinical use of granulocyte-colony stimulating factor. Br J Hosp Med (Lond). 2014 Feb;75(2):C29-32.  [Content Brief]

  [3]. Panopoulos AD, et al. Granulocyte colony-stimulating factor: molecular mechanisms of action during steady state and 'emergency' hematopoiesis. Cytokine. 2008 Jun;42(3):277-88.  [Content Brief]