Galectin-1/LGALS1 蛋白, Human

Galectin-1/LGALS1 is a lectin that binds β-galactoside and complex carbohydrates and regulates apoptosis, proliferation, and differentiation. It inhibits CD45 phosphatase activity, blocks Lyn kinase dephosphorylation, and induces T cell apoptosis. Galectin-1/LGALS1 Protein, Human is the recombinant human-derived Galectin-1/LGALS1 protein, expressed by E. coli , with tag free.

Galectin-1/LGALS1 蛋白作为一种凝集素，能够结合 β-半乳糖苷和多种复杂碳水化合物。它在细胞凋亡、细胞增殖和细胞分化的调节中起着关键作用。Galectin-1/LGALS1 通过抑制 CD45 的蛋白磷酸酶活性发挥作用，从而阻碍 Lyn 激酶的去磷酸化。此外，它还是 T 细胞凋亡的有效诱导剂。Galectin-1/LGALS1 以同二聚体形式存在，与多种细胞实体形成相互作用，包括 CD2、CD3、CD4、CD6、CD7、CD43、ALCAM 和 CD45。它还结合 LGALS3BP、层粘连蛋白（通过聚-N-乙酰基乳糖胺）和 SUSD2。值得注意的是，Galectin-1/LGALS1 与货物受体 TMED10 相互作用，促进从细胞质易位到内质网-高尔基中间室 (ERGIC) 和随后的分泌。

1.Measured by its ability to agglutinate human red blood cells and the ED₅₀ is typically 3-30μg/mL.
2.Measured in a cell proliferation assay using ARPE-19 cells. The ED₅₀ for this effect is 0.2112-0.3052 μg/mL.

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  Measured in a cell proliferation assay using ARPE-19 cells. The ED₅₀ for this effect is 0.3052 μg/mL, corresponding to a specific activity is 3276.54 units/mg.

Human

E. coli

Tag Free

P09382/NP_002296.1 (A2-D135)

3956  [NCBI]

ACGLVASNLNLKPGECLRVRGEVAPDAKSFVLNLGKDSNNLCLHFNPRFNAHGDANTIVCNSKDGGAWGTEQREAVFPFQPGSVAEVCITFDQANLTVKLPDGYEFKFPNRLNLEAINYMAADGDFKIKCVAFD

Approximately 15 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.