Galectin-3/LGALS3 蛋白, Mouse (HEK293, His)

Galectin-3/LGALS3 protein binds IgE and cooperates with integrins α-3 and β-1 to promote endothelial cell migration.It contributes to epithelial cell differentiation and acts as a splicing factor in the nucleus.Galectin-3/LGALS3 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Galectin-3/LGALS3 protein, expressed by HEK293 , with C-His labeled tag.

Galectin-3 (LGALS3) 是一种半乳糖特异性凝集素，能够结合 IgE，并与 α-3、β-1 整合素协同作用，参与 CSPG4 诱导的内皮细胞迁移刺激。与 DMBT1 一起，它对于早期胚胎发生过程中柱状上皮细胞的终末分化至关重要。在细胞核中，Galectin-3 充当前体 mRNA 剪接因子。在细胞核外，它在急性炎症反应中发挥着至关重要的作用，包括中性粒细胞活化和粘附、单核巨噬细胞趋化、凋亡中性粒细胞的调理作用和肥大细胞活化。此外，与 TRIM16 配合，Galectin-3 有助于识别膜损伤，动员核心自噬调节因子 ATG16L1 和 BECN1 响应受损的内膜。该蛋白可能形成同源或异源二聚体，并与各种伙伴相互作用，包括 DMBT1、CD6、ALCAM、ITGA3、ITGB1、CSPG4、LGALS3BP、LYPD3、ZFTRAF1 和 UACA，并与 TRIM16 相互作用介导受损内膜的自噬。

Measured by the ability of the immobilized protein to support the adhesion of CTLL-2 cells. The ED₅₀ for this effect is 1.044 μg/mL, corresponding to a specific activity is 957.854 units/mg.

Mouse

HEK293

C-His

P16110 (A2-I264)

16854  [NCBI]

ADSFSLNDALAGSGNPNPQGYPGAWGNQPGAGGYPGAAYPGAYPGQAPPGAYPGQAPPGAYPGQAPPSAYPGPTAPGAYPGPTAPGAYPGQPAPGAFPGQPGAPGAYPQCSGGYPAAGPYGVPAGPLTVPYDLPLPGGVMPRMLITIMGTVKPNANRIVLDFRRGNDVAFHFNPRFNENNRRVIVCNTKQDNNWGKEERQSAFPFESGKPFKIQVLVEADHFKVAVNDAHLLQYNHRMKNLREISQLGISGDITLTSANHAMI

Approximately 35-50 kDa

Greater than 95% as determined by Tris-Bis PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of PBS, 300 mM L-Arginine, pH 7.4 or 50 mM HEPES, 150 mM NaCl, 5 mM EDTA, 2 mM TCEP, pH 7.4 or PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.