GAPDH 蛋白, Mouse (His)

The GAPDH protein has dual roles in glycolysis and nuclear function. It also affects the organization and assembly of the cytoskeleton and promotes the association of microtubules and membranes. GAPDH Protein, Mouse (His) is the recombinant mouse-derived GAPDH protein, expressed by E. coli , with N-6*His labeled tag. The total length of GAPDH Protein, Mouse (His) is 333 a.a., with molecular weight of ~38 kDa.

GAPDH 蛋白具有双重功能，具有 3-磷酸甘油醛脱氢酶和亚硝基酶活性，分别在糖酵解和核功能中发挥着不可或缺的作用。在糖酵解中，它作为关键酶催化初始步骤，将 D-甘油醛 3-磷酸 (G3P) 转化为 3-磷酸-D-甘油酰磷酸。除了其代谢作用之外，GAPDH 还调节细胞骨架的组织和组装，并通过刺激 CHP1 与微管的结合（通过相似性）促进 CHP1 依赖性微管和膜的关联。它也是步态复合体的一个组成部分，在炎症过程中介导干扰素γ诱导的转录选择性翻译抑制。在先天免疫中，GAPDH 分别通过与 TRAF2 和 TRAF3 相互作用（通过相似性）促进 TNF 诱导的 NF-κ-B 激活和 I 型干扰素产生。此外，它参与核事件，包括转录、RNA 转运、DNA 复制和细胞凋亡，可能是由其亚硝基酶活性介导的，导致核靶蛋白如 SIRT1、HDAC2 和 PRKDC 发生半胱氨酸 S-亚硝基化（通过相似性）。GAPDH 的多方面功能强调了其在细胞过程中的多功能和关键作用。

The specific activity of GAPDH was determined to be 947.65 U/mg.

Mouse

E. coli

N-6*His

P16858 (M1-E333)

14433  [NCBI]

MVKVGVNGFGRIGRLVTRAAICSGKVEIVAINDPFIDLNYMVYMFQYDSTHGKFNGTVKAENGKLVINGKPITIFQERDPTNIKWGEAGAEYVVESTGVFTTMEKAGAHLKGGAKRVIISAPSADAPMFVMGVNHEKYDNSLKIVSNASCTTNCLAPLAKVIHDNFGIVEGLMTTVHAITATQKTVDGPSGKLWRDGRGAAQNIIPASTGAAKAVGKVIPELNGKLTGMAFRVPTPNVSVVDLTCRLEKPAKYDDIKKVVKQASEGPLKGILGYTEDQVVSCDFNSNSHSSTFDAGAGIALNDNFVKLISWYDNEYGYSNRVVDLMAYMASKE

Approximately 38 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.