GBA/Glucosylceramidase 蛋白, Human (HEK293, His)

GBA (glucosylceramidase) is a lysosomal enzyme that degrades lipids and aids in cell membrane turnover. It hydrolyzes glucosylceramide to form ceramide and glucose. GBA/Glucosylceramidase Protein, Human (HEK293, His) is the recombinant human-derived GBA/Glucosylceramidase protein, expressed by HEK293 , with C-His labeled tag.

GBA（葡萄糖神经酰胺酶）是位于溶酶体区室中的一种重要酶，在复杂脂质的降解和细胞膜更新中发挥着核心作用。它催化葡萄糖神经酰胺 (GlcCers)（例如 β-D-葡萄糖基-(1<->1')-N-酰基鞘-4-烯氨酸）水解成游离神经酰胺和葡萄糖。这种酶活性对于 PKC 激活的神经酰胺形成挽救途径至关重要。此外，GBA 参与转糖基反应，将葡萄糖从 GlcCer 转移到胆固醇，这是含有单不饱和脂肪酸的 GlcCer 的首选过程。在特定条件下，GBA可以催化逆反应，将葡萄糖从胆固醇3-β-D-葡萄糖苷转移到神经酰胺。此外，GBA可以水解胆固醇3-β-D-葡萄糖苷，产生葡萄糖和胆固醇。虽然 GBA 对半乳糖神经酰胺 (GalCers) 的活性较低，但它也可以在体外在 GalCers 和胆固醇之间转移半乳糖。相比之下，木糖神经酰胺 (XylCer) 不是水解的重要底物，但它是转木糖基活性的良好木糖供体，形成胆固醇 3-β-D-木糖苷。

Measured by its ability to hydrolyze 4-methylumbelliferyl-beta-D-glucopyranoside(HY-123633). The specific activity is 286- 542 pmol/min/μg that incubate at 37°C for 20 minutes.

Human

HEK293

C-His

P04062-1/NP_000148.2 (A40-Q536)

2629  [NCBI]

ARPCIPKSFGYSSVVCVCNATYCDSFDPPTFPALGTFSRYESTRSGRRMELSMGPIQANHTGTGLLLTLQPEQKFQKVKGFGGAMTDAAALNILALSPPAQNLLLKSYFSEEGIGYNIIRVPMASCDFSIRTYTYADTPDDFQLHNFSLPEEDTKLKIPLIHRALQLAQRPVSLLASPWTSPTWLKTNGAVNGKGSLKGQPGDIYHQTWARYFVKFLDAYAEHKLQFWAVTAENEPSAGLLSGYPFQCLGFTPEHQRDFIARDLGPTLANSTHHNVRLLMLDDQRLLLPHWAKVVLTDPEAAKYVHGIAVHWYLDFLAPAKATLGETHRLFPNTMLFASEACVGSKFWEQSVRLGSWDRGMQYSHSIITNLLYHVVGWTDWNLALNPEGGPNWVRNFVDSPIIVDITKDTFYKQPMFYHLGHFSKFIPEGSQRVGLVASQKNDLDAVALMHPDGSAVVVVLNRSSKDVPLTIKDPAVGFLETISPGYSIHTYLWRRQ

Approximately 60-80 kDa due to the glycosylation.

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or PBS, pH 8.0, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.