2. 重组蛋白
  3. Enzymes & Regulators
  4. Oxidoreductases (EC 1)
  5. GCDH 蛋白, Human (sf9, His)

GCDH 蛋白, Human (sf9, His)

目录号: HY-P76357
产品使用指南

GCDH 蛋白对于 L-赖氨酸、L-羟赖氨酸和 L-色氨酸的降解至关重要。它催化戊二酰辅酶A氧化脱羧为巴豆酰辅酶A，释放CO(2)。GCDH 蛋白, Human (sf9, His) 是重组的 GCDH 蛋白，由 Sf9 insect cells 表达，带有 C-His 标签。

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100 μg   询价  

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描述

The GCDH protein is essential for the degradation of L-lysine, L-hydroxylysine, and L-tryptophan. It catalyzes the oxidative decarboxylation of glutaryl-CoA to crotonyl-CoA, releasing CO(2). GCDH Protein, Human (sf9, His) is the recombinant human-derived GCDH protein, expressed by Sf9 insect cells , with C-His labeled tag.
研究背景

GCDH 蛋白通过催化戊二酰辅酶 A 氧化脱羧为巴豆酰辅酶 A，并伴随 CO(2) 的释放，在 L-赖氨酸、L-羟赖氨酸和 L-色氨酸代谢降解途径中发挥至关重要的作用。这种酶促过程涉及利用电子转移黄素蛋白作为其电子受体，强调了蛋白质在代谢途径中有效发挥功能对这种辅助因子的依赖。值得注意的是，GCDH 蛋白的短亚型是无活性的，表明在调节该代谢过程中存在具有不同功能特性的多种亚型。
种属

Human
表达系统

Sf9 insect cells
标签

C-His
蛋白编号

Q92947-1/NP_000150.1 (R45-K438)
基因 ID
蛋白结构
N-term
GCDH (R45-K438)
Accession # Q92947-1/NP_000150.1
His
C-term
同用名
Glutaryl-CoA Dehydrogenase Mitochondrial; GCD; GCDH
分子量

Approximately 44.9 kDa.
纯度

Greater than 95% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

GCDH Protein, Human (sf9, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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