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  3. GCSH 蛋白, Human (His)

GCSH 蛋白, Human (His)

目录号: HY-P76358
COA 产品使用指南

GCSH 蛋白是甘氨酸裂解系统的重要组成部分,可有效地将甘氨酸的甲胺基团从 P 蛋白 (GLDC) 穿梭至 T 蛋白 (GCST),从而促进甘氨酸降解。GCSH 蛋白, Human (His) 是重组的 GCSH 蛋白,由 E. coli 表达,带有 N-His 标签。GCSH 蛋白, Human (His) 全长 125 个氨基酸,分子量约为 ~17 KDa。

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规格 价格 是否有货 数量
Free Sample   Apply now  
5 μg ¥550 In-stock
10 μg ¥950 In-stock
50 μg ¥2650 In-stock
100 μg ¥4500 In-stock
500 μg ¥12600 询价
1 mg ¥21400 询价
> 1 mg   询价  

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描述

GCSH protein, a vital component of the glycine cleavage system, efficiently shuttles the methylamine group of glycine from the P protein (GLDC) to the T protein (GCST), contributing to glycine degradation. GCSH Protein, Human (His) is the recombinant human-derived GCSH protein, expressed by E. coli , with N-His labeled tag. The total length of GCSH Protein, Human (His) is 125 a.a., with molecular weight of ~17 KDa.

研究背景

甘氨酸裂解系统由甘氨酸裂解系统 H 蛋白 (GCSH) 促进,是甘氨酸降解不可或缺的一部分。在此过程中,GCSH 通过有效地将甘氨酸的甲胺基团从 P 蛋白 (GLDC) 穿梭到 T 蛋白 (GCST) 发挥着至关重要的作用。

种属

Human

表达系统

E. coli

标签

N-His

蛋白编号

P23434 (S49-E173)

基因 ID
蛋白结构
N-term
His
GCSH (S49-E173)
Accession # P23434
C-term
同用名
Glycine cleavage system H protein, mitochondrial; Lipoic acid-containing protein; GCSH
氨基酸序列

SVRKFTEKHEWVTTENGIGTVGISNFAQEALGDVVYCSLPEVGTKLNKQDEFGALESVKAASELYSPLSGEVTEINEALAENPGLVNKSCYEDGWLIKMTLSNPSELDELMSEEAYEKYIKSIEE

分子量

Approximately 17 kDa.

纯度

Greater than 95% as determined by reducing SDS-PAGE

性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

GCSH Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
GCSH Protein, Human (His)
目录号:
HY-P76358
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