GFRA1/GDNFR-alpha-1 蛋白, Human (HEK293, His)

The GFRA1/GDNFR-alpha-1 Protein serves as a receptor for GDNF, facilitating GDNF-induced autophosphorylation and activation of the RET receptor. In a complex, two molecules of GDNFR-alpha-1 are believed to associate with the disulfide-linked GDNF dimer and two molecules of RET. It interacts with RET and SORL1, either alone or in a complex with GDNF, leading to GFRA1 internalization without degradation. GFRA1/GDNFR-alpha-1 Protein, Human (HEK293, His) is the recombinant human-derived GFRA1/GDNFR-alpha-1 protein, expressed by HEK293 , with C-6*His labeled tag.

GFRA1/GDNFR-α-1 是胶质细胞系衍生神经营养因子 (GDNF) 的受体，在介导 GDNF 诱导的自身磷酸化和 RET 受体激活中发挥着至关重要的作用。所提出的模型表明，两个 GDNFR-α-1 分子与二硫键连接的 GDNF 二聚体和两个 RET 分子形成复合物。相互作用研究证实与 RET 直接结合，促进 GDNF 信号传导。此外，GFRA1 单独或与 GDNF 复合地与 SORL1 结合，导致 GFRA1 内化而不降解。这种复杂的相互作用凸显了 GDNF 介导的细胞反应中涉及的调节机制，揭示了 GFRA1 在协调对细胞稳态和发育至关重要的信号事件中的多方面作用。

Human

HEK293

C-6*His

P56159-2 (D25-K429)

2674  [NCBI]

DRLDCVKASDQCLKEQSCSTKYRTLRQCVAGKETNFSLASGLEAKDECRSAMEALKQKSLYNCRCKRGMKKEKNCLRIYWSMYQSLQGNDLLEDSPYEPVNSRLSDIFRVVPFISVEHIPKGNNCLDAAKACNLDDICKKYRSAYITPCTTSVSNDVCNRRKCHKALRQFFDKVPAKHSYGMLFCSCRDIACTERRRQTIVPVCSYEEREKPNCLNLQDSCKTNYICRSRLADFFTNCQPESRSVSSCLKENYADCLLAYSGLIGTVMTPNYIDSSSLSVAPWCDCSNSGNDLEECLKFLNFFKDNTCLKNAIQAFGNGSDVTVWQPAFPVQTTTATTTTALRVKNKPLGPAGSENEIPTHVLPPCANLQAQKLKSNVSGNTHLCISNGNYEKEGLGASSHITTK

Approximately 60.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.