GFRA1/GDNFR-alpha-1 蛋白, Mouse (HEK293, His)

GFRA1/GDNFR-alpha-1, a receptor for GDNF, mediates GDNF-induced autophosphorylation and activation of the RET receptor. It interacts with RET and forms a complex with disulfide-linked GDNF dimer, and interacts with SORL1. The SORL1-GFRA1 interaction results in GFRA1 internalization without degradation. GFRA1/GDNFR-alpha-1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived GFRA1/GDNFR-alpha-1 protein, expressed by HEK293 , with C-His labeled tag. The total length of GFRA1/GDNFR-alpha-1 Protein, Mouse (HEK293, His) is 401 a.a., with molecular weight of 55-65 KDa.

GFRA1，称为 GDNFR-α-1，作为神经胶质细胞源性神经营养因子 (GDNF) 的关键受体发挥作用。其功能意义在于介导 GDNF 诱导的自磷酸化和 RET 受体的激活。在这种分子相互作用中，两个 GDNFR-α 分子被认为与二硫键连接的 GDNF 二聚体错综复杂地结合，与两个 RET 分子一起形成复合物。这种与 RET 的相互作用强调了 GFRA1 在协调对细胞反应至关重要的信号级联中的作用。此外，GFRA1 在其相互作用中表现出额外的复杂性，与 SORL1 形成复合物，无论是独立的还是与 GDNF 一起形成的。值得注意的是，SORL1 和 GFRA1 之间的相互作用导致 GFRA1 内化而不降解，为其细胞功能增加了一层复杂的调控。

Immobilized Mouse GNDF at 25 ng/mL (100 μL/well) can bind Mouse GFRA1/GDNFR-alpha-1 Protein. The ED₅₀ for this effect is 0.3750 μg/mL.

• 
  Immobilized Mouse GNDF at 25 ng/mL (100 μL/well) can bind Mouse GFRA1/GDNFR-alpha-1 Protein. The ED₅₀ for this effect is 0.3750 μg/mL.

Mouse

HEK293

C-His

P97785-2 (D25-S425)

14585  [NCBI]

DRLDCVKASDQCLKEQSCSTKYRTLRQCVAGKETNFSLTSGLEAKDECRSAMEALKQKSLYNCRCKRGMKKEKNCLRIYWSMYQSLQGNDLLEDSPYEPVNSRLSDIFRAVPFISVEHISKGNNCLDAAKACNLDDTCKKYRSAYITPCTTSMSNEVCNRRKCHKALRQFFDKVPAKHSYGMLFCSCRDVACTERRRQTIVPVCSYEERERPNCLNLQDSCKTNYICRSRLADFFTNCQPESRSVSNCLKENYADCLLAYSGLIGTVMTPNYIDSSSLSVAPWCDCSNSGNDLEDCLKFLNFFKDNTCLKNAIQAFGNGSDVTMWQPAPPVQTTTATTTTAFRIKNKPLGPAGSENEIPTHVLPPCANLQAQKLKSNVSGSTHLCLSDNDYGKDGLAGASS

55-65 kDa.

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.