GIP 蛋白, Human (HEK293, hFc)

The GIP protein is a member of the glucagon superfamily, an important incretin hormone that stimulates pancreatic beta cells to secrete insulin in response to food intake. Through its G protein-coupled receptor, it activates adenylyl cyclase and other signal transduction pathways. GIP Protein, Human (HEK293, hFc) is the recombinant human-derived GIP protein, expressed by HEK293 , with C-hFc labeled tag. The total length of GIP Protein, Human (HEK293, hFc) is 72 a.a., with molecular weight of ~38.77 kDa.

GIP 蛋白属于胰高血糖素超家族，是一种对葡萄糖稳态至关重要的肠促胰岛素激素。它的重要性在于它是胰腺β细胞响应食物摄入和营养吸收而分泌胰岛素的有效刺激剂。这种刺激是通过激活其 G 蛋白偶联受体、触发腺苷酸环化酶和其他信号转导途径而发生的。虽然 GIP 是一种相对较差的胃酸分泌抑制剂，但其在胰岛素调节中的主要作用使其成为代谢过程中的关键参与者。该基因表现出偏向表达，在十二指肠 (RPKM 96.0) 和小肠 (RPKM 33.9) 中检测到显着水平，强调其参与胃肠道内的消化和代谢功能。

Immobilized  Human GIP at 0.5 μg/mL (100 μL/well) can bind Anti-GIP Antibody, The ED₅₀ for this effect is 82.46 ng/mL.

Human

HEK293

C-hFc

NP_004114.1 (E22-Q93)

2695  [NCBI]

EKKEGHFSALPSLPVGSHAKVSSPQPRGPRYAEGTFISDYSIAMDKIHQQDFVNWLLAQKGKKNDWKHNITQ

Approximately 38.77 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.