1. 重组蛋白
  2. Receptor Proteins
  3. GIPR 蛋白, Mouse (HEK293, His)

GIPR 蛋白, Mouse (HEK293, His)

目录号: HY-P702801
COA 产品使用指南

AK2 蛋白通过催化 ATP 和 AMP 之间末端磷酸基团的可逆转移,在细胞能量稳态和腺嘌呤核苷酸代谢中发挥关键作用。其腺苷酸激酶活性在调节磷酸盐利用和从头 AMP 生物合成途径中发挥着关键作用,有助于细胞能量资源的复杂平衡。GIPR 蛋白, Mouse (HEK293, His) 是重组的 GIPR 蛋白,由 HEK293 表达,带有 C-10*His 标签。GIPR 蛋白, Mouse (HEK293, His) 全长 116 个氨基酸

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规格 价格 是否有货 数量
20 μg ¥2300 In-stock
50 μg ¥4350 In-stock
100 μg ¥7000 询价
> 100 μg   询价  

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

AK2 protein plays a key role in cellular energy homeostasis and adenine nucleotide metabolism by catalyzing the reversible transfer of terminal phosphate groups between ATP and AMP. Its adenylate kinase activity plays a key role in regulating phosphate utilization and de novo AMP biosynthetic pathways, contributing to the complex balance of cellular energy resources. GIPR Protein, Mouse (HEK293, His) is the recombinant mouse-derived GIPR, expressed by HEK293 , with C-10*His labeled tag. The total length of GIPR Protein, Mouse (HEK293, His) is 116 a.a.,

研究背景

腺苷酸激酶 2 (AK2) 是一种催化 ATP 和 AMP 之间末端磷酸基团可逆转移的酶,这是细胞能量稳态和腺嘌呤核苷酸代谢的关键反应。AK2 的腺苷酸激酶活性对于调节磷酸盐利用和 AMP 的从头生物合成(细胞能量管理的关键过程)至关重要。除了在能量代谢中的作用之外,AK2 在造血中也发挥着重要作用,强调了它在血细胞发育中的重要性。AK2 的多方面功能强调了其在维持细胞能量平衡、核苷酸合成和支持造血等基本生物过程中的关键作用。参考资料:所提供的信息基于截至 2022 年 1 月的一般知识,未引用具体参考资料。

生物活性

Measured by its binding ability in a functional ELISA. Immobilized GIPR, Mouse (HEK293, His) at 2 μg/mL can bind Anti-Mouse Gipr Recombinant Antibody, the EC50 is ≤30 ng/mL.

种属

Mouse

表达系统

HEK293

标签

C-10*His

蛋白编号

P54819-1 (E19-Q134)

基因 ID

381853

同用名
Gastric inhibitory polypeptide receptor; Glucose-dependent insulinotropic polypeptide receptor
分子量

32 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

性状

无菌粉末

组分

Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0. or PBS, 6% Trehalose, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

GIPR Protein, Mouse (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
GIPR Protein, Mouse (HEK293, His)
目录号:
HY-P702801
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