GM-CSF R alpha 蛋白, Human (HEK293, Fc)

GM-CSF R alpha is the alpha subunit of the heterodimeric receptor for colony stimulating factor 2. GM-CSF R alpha binds to the cytokine with high specificity and low affinity^[1]. GM-CSF R alpha binds to GM-CSF and induces cell differentiation^[2]. GM-CSF R alpha monoclonal antibody decreases GM-CSFRα expression on GM-CSF-responsive cells and shows anti-inflammatory activity in rheumatoid arthritis (RA)^[3]. GM-CSF R alpha Protein, Human (HEK293, Fc) is a recombinant protein with a C-Terminal Fc label, It consists of 320 amino acids (M1-G320) and is produced in HEK293 cells.

GM-CSF R α 在骨髓细胞和一些非造血细胞（例如内皮细胞）上表达，但在 T 细胞上不表达^[2]。
人 GM-CSF R α 的氨基酸序列CSF R α 蛋白与小鼠 GM-CSF R α 蛋白同源性较低。
GM-CSF 受体（GM-CSFR）由两个亚基组成，一个是低亲和力结合细胞因子的 α 亚基，另一个是较大的 α 亚基。β亚基（β常见；βc），负责信号传导，形成三元受体复合物。对细胞因子白细胞介素 (IL)-3 和 IL-5 的信号转导也由 βc 介导；因此，受体特异性归因于 GM-CSFRα^[1]。GM-CSF 与其受体结合后，Janus-kinase-2 (JAK-2) 被募集至 β 链的胞质结构域，并发生 JAK-2 激活，随后诱导 STAT-5 磷酸化。该信号通路诱导STAT-5二聚体向细胞核迁移，促进pim-1、CIS等多种基因转录，诱导细胞分化^[2]。
GM-CSFR α -亚基显着增加 RA 滑膜中的阳性滑膜巨噬细胞。GM-CSFR α 单克隆抗体可抑制小鼠胶原诱导的关节炎模型中的疾病活动^[3]。

Measured by its ability to inhibit GM-CSF dependent proliferation of TF-1 human erythroleukemic cells. The ED₅₀ for this effect is typically <15 μg/mL.

Human

HEK293

C-hFc

P15509 (E23-G320)

1438  [NCBI]

EKSDLRTVAPASSLNVRFDSRTMNLSWDCQENTTFSKCFLTDKKNRVVEPRLSNNECSCTFREICLHEGVTFEVHVNTSQRGFQQKLLYPNSGREGTAAQNFSCFIYNADLMNCTWARGPTAPRDVQYFLYIRNSKRRREIRCPYYIQDSGTHVGCHLDNLSGLTSRNYFLVNGTSREIGIQFFDSLLDTKKIERFNPPSNVTVRCNTTHCLVRWKQPRTYQKLSYLDFQYQLDVHRKNTQPGTENLLINVSGDLENRYNFPSSEPRAKHSVKIRAADVRILNWSSWSEAIEFGSDDG

Approximately 90 kDa

Greater than 90% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Hansen G, et al. The structure of the GM-CSF receptor complex reveals a distinct mode of cytokine receptor activation. Cell. 2008 Aug 8;134(3):496-507.  [Content Brief]

  [2]. Lotfi N, et al. Roles of GM-CSF in the Pathogenesis of Autoimmune Diseases: An Update. Front Immunol. 2019 Jun 4;10:1265.  [Content Brief]

  [3]. Cook AD, et al. Granulocyte macrophage colony-stimulating factor receptor α expression and its targeting in antigen-induced arthritis and inflammation. Arthritis Res Ther. 2016 Dec 1;18(1):287.  [Content Brief]