1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Neurotrophic Factors
  4. GMFG 蛋白, Human (His)

GMFG 蛋白, Human (His)

目录号: HY-P76367
COA 产品使用指南

GMFG 蛋白主要在肺、心脏和胎盘中表达,强调了其在这些重要组织中的重要作用。作为 GMF 亚家族内肌动蛋白结合蛋白 ADF 家族的成员,GMFG 参与与肌动蛋白动力学相关的细胞过程。GMFG 蛋白, Human (His) 是重组的 GMFG 蛋白,由 E. coli 表达,带有 N-6*His 标签。GMFG 蛋白, Human (His) 全长 141 个氨基酸,分子量约为 ~18 kDa。

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10 μg ¥1050 In-stock
50 μg ¥3000 In-stock
100 μg ¥5000 In-stock
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描述

GMFG protein shows predominant expression in the lung, heart, and placenta, emphasizing its important role in these important tissues. As a member of the ADF family of actin-binding proteins within the GMF subfamily, GMFG is involved in cellular processes related to actin dynamics. GMFG Protein, Human (His) is the recombinant human-derived GMFG protein, expressed by E. coli , with N-6*His labeled tag. The total length of GMFG Protein, Human (His) is 141 a.a., with molecular weight of ~18 kDa.

研究背景

GMFG 蛋白属于 GMF 亚家族中的肌动蛋白结合蛋白 ADF 家族,主要在特定组织中表达,主要存在于肺、心脏和胎盘中。作为肌动蛋白解聚因子 (ADF) 家族的一员,GMFG 可能参与调节肌动蛋白动力学,这是细胞结构、运动和各种细胞内活动所必需的关键过程。重要器官中的独特表达模式表明GMFG在细胞迁移、组织发育或肺、心脏和胎盘特有的生理功能等过程中的潜在作用。对 GMFG 在这些组织内的精确分子功能和相互作用的进一步研究可以为了解其对细胞动力学和器官特异性过程的贡献提供有价值的见解。

种属

Human

表达系统

E. coli

标签

N-6*His

蛋白编号

O60234 (S2-R142)

基因 ID
蛋白结构
N-term
6*His
GMFG (S2-R142)
Accession # O60234
C-term
同用名
Glia maturation factor gamma; GMF-gamma; GMFG
氨基酸序列

SDSLVVCEVDPELTEKLRKFRFRKETDNAAIIMKVDKDRQMVVLEEEFQNISPEELKMELPERQPRFVVYSYKYVHDDGRVSYPLCFIFSSPVGCKPEQQMMYAGSKNRLVQTAELTKVFEIRTTDDLTEAWLQEKLSFFR

分子量

Approximately 18 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween 80.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

GMFG Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
GMFG Protein, Human (His)
目录号:
HY-P76367
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