GNMT 蛋白, Human (His)

Glycine N-methyltransferase (GNMT) is responsible for catalyzing the methylation of glycine, using S-adenosylmethionine (AdoMet) to generate N-methylglycine (sarcosine), and simultaneously producing S-adenosyl homocysteine AdoHcy. This reaction is complexly regulated by 5-methyltetrahydrofolate binding, highlighting the critical role of GNMT in the regulation of methyl metabolism. GNMT Protein, Human (His) is the recombinant human-derived GNMT protein, expressed by E. coli , with N-6*His labeled tag.

甘氨酸 N-甲基转移酶 (GNMT) 是一种使用 S-腺苷甲硫氨酸 (AdoMet) 作为甲基供体催化甘氨酸甲基化的酶，从而形成 N-甲基甘氨酸（肌氨酸）和 S-腺苷高半胱氨酸 (AdoHcy)。该反应对于甲基代谢的调节至关重要，因为 GNMT 在维持 S-腺苷-L-蛋氨酸和 S-腺苷-L-同型半胱氨酸水平之间的平衡中发挥着关键作用。该酶参与一碳代谢的调节，并有助于控制细胞甲基化电位。5-甲基四氢叶酸的结合涉及调节 GNMT 的活性，突出了其在协调甲基利用和稳态中的作用。

Human

E. coli

N-6*His

Q14749 (M1-D295)

27232  [NCBI]

MVDSVYRTRSLGVAAEGLPDQYADGEAARVWQLYIGDTRSRTAEYKAWLLGLLRQHGCQRVLDVACGTGVDSIMLVEEGFSVTSVDASDKMLKYALKERWNRRHEPAFDKWVIEEANWMTLDKDVPQSAEGGFDAVICLGNSFAHLPDCKGDQSEHRLALKNIASMVRAGGLLVIDHRNYDHILSTGCAPPGKNIYYKSDLTKDVTTSVLIVNNKAHMVTLDYTVQVPGAGQDGSPGLSKFRLSYYPHCLASFTELLQAAFGGKCQHSVLGDFKPYKPGQTYIPCYFIHVLKRTD

31&80 kDa

Greater than 95% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.