GPNMB/Osteoactivin 蛋白, Human (HEK293, His)

GPNMB, also known as osteoactivin, is a protein thought to have a potential role as a melanin-producing enzyme. Melanogenic enzymes are involved in the production of melanin, the pigment that determines skin, hair and eye color. GPNMB/Osteoactivin Protein, Human (HEK293, His) is the recombinant human-derived GPNMB/Osteoactivin protein, expressed by HEK293 , with C-6*His labeled tag. The total length of GPNMB/Osteoactivin Protein, Human (HEK293, His) is 465 a.a., with molecular weight of 80-120 kDa.

GPNMB，也称为骨激活素，是一种被认为具有黑色素生成酶潜在作用的蛋白质。黑色素生成酶参与黑色素的产生，黑色素是决定皮肤、头发和眼睛颜色的色素。虽然 GPNMB 在黑色素合成中的确切作用机制尚不完全清楚，但研究表明它参与了这一过程。需要进一步的研究来阐明 GPNMB 在黑色素生成中的具体功能和调节机制，并确定其在黑色素生成中的意义。

Measured in a cell proliferation assay using HUVEC cells. The ED₅₀ this effect is 1.704 μg/mL, corresponding to a specific activity is 5.86×10³ units/mg.

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  Measured in a cell proliferation assay using HUVEC cells. The ED₅₀ for this effect is 1.704 μg/mL, corresponding to a specific activity is 5.86×10³ units/mg.

Human

HEK293

C-6*His

Q14956 (A22-P486)

10457  [NCBI]

AKRFHDVLGNERPSAYMREHNQLNGWSSDENDWNEKLYPVWKRGDMRWKNSWKGGRVQAVLTSDSPALVGSNITFAVNLIFPRCQKEDANGNIVYEKNCRNEAGLSADPYVYNWTAWSEDSDGENGTGQSHHNVFPDGKPFPHHPGWRRWNFIYVFHTLGQYFQKLGRCSVRVSVNTANVTLGPQLMEVTVYRRHGRAYVPIAQVKDVYVVTDQIPVFVTMFQKNDRNSSDETFLKDLPIMFDVLIHDPSHFLNYSTINYKWSFGDNTGLFVSTNHTVNHTYVLNGTFSLNLTVKAAAPGPCPPPPPPPRPSKPTPSLATTLKSYDSNTPGPAGDNPLELSRIPDENCQINRYGHFQATITIVEGILEVNIIQMTDVLMPVPWPESSLIDFVVTCQGSIPTEVCTIISDPTCEITQNTVCSPVDVDEMCLLTVRRTFNGSGTYCVNLTLGDDTSLALTSTLISVP

80-120 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2 or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.